化学动力治疗纳米药物的类Fenton催化性能评价实验设计

Experiment design for evaluation of Fenton-like reaction catalytic performance of chemodynamic therapy nanomedicines

纳米医药是当前最前沿的科学研究方向之一,但药学专业的实验课程却很少涉及相关内容。化学动力治疗是一种新型肿瘤疗法,其利用纳米材料催化Fenton反应生成羟基自由基(·OH)实现抗癌效果。该文根据培养目标设计了紫外-可见分光光度法和荧光分析法两种方法,来评估Cu基纳米药物的类Fenton催化性能。结果显示,Cu2O纳米粒子在与肿瘤微环境相似的微酸性条件下,具备催化类Fenton反应产生·OH的能力。该实验非常适合药学专业仪器分析实验课程,既能让学生掌握实验技能,又能帮助学生了解科研前沿动态,培养其创新思维和科学素养。

Nanomedicine is one of the most frontiers of scientific research, but few experimental courses in pharmacy major involve the related content. Chemodynamic therapy is a new type of tumor therapy, which utilizes nanomaterials to catalyze Fenton reactions to generate hydroxyl radicals(·OH) to achieve anticancer effects.According to the training objectives, two methods, UV-Vis spectrophotometry and fluorescence analysis are designed to evaluate the Fenton-like catalytic performance of Cu based nanomedicines. The results show that Cu_(2)O nanoparticles have the ability to catalyze Fenton-like reaction to produce ·OH under slightly acidic conditions similar to the tumor microenvironment. This experiment is very suitable for the instrumental analysis experiment course of pharmacy major. It not only enables students to master experimental skills, but also helps students understand the frontier trends of scientific research and cultivate their innovative thinking and scientific literacy.