摘要
结核分枝杆菌(Mycobacterium tuberculosis,MTB)作为引发结核病的主要病原体,其遗传多态性相对保守,随着二代全基因组测序技术的发展和普及,在不同宿主甚至同一宿主内都可观察到MTB菌株丰富的遗传异质性。宿主内异质性的成因包括不同菌株的多重(混合)感染和同一菌株的微进化。宿主内异质性的发生机制及其对MTB耐药、传播和诊疗等方面的影响及应用尚未完全清楚。本文综述了MTB宿主内遗传异质性的鉴定及相关研究进展,以期为结核病防控工作提供更多新思路。
Tuberculosis(TB)is an infectious disease caused by the Mycobacterium tuberculosis(MTB).The genetic polymorphism of the MTB complex is relatively conservative.With the impressive progress in the field of next generation sequencing(NGS)and the whole-genome sequencing(WGS)analysis,vast genetic heterogeneity has been observed in MTB isolates from different hosts and even within the same host.The reasons for this phenomenon are complex,at least including multiple or mixed infections of different strains and microevolution of the same strain within the host.Still,its mechanism and role in drug resistance,transmission,diagnosis and treatment of TB are not fully understood.This article reviews the identification and current research progress of the within-host genetic heterogeneity of MTB.
作者
张睿
刘艳萍
钱军
方强林
杨崇广
Zhang Rui;Liu Yanping;Qian Jun;Fang Qianglin;Yang Chongguang(School of Public Health(Shenzhen),Shenzhen Campus of Sun Yat-sen University,Shenzhen 518107,China)
出处
《中国防痨杂志》
CAS
CSCD
2022年第11期1199-1204,共6页
Chinese Journal of Antituberculosis
基金
深圳南山区高水平医学团队项目(SZSM202103008)。
关键词
分枝杆菌
结核
遗传异质性
全基因组测序
感染
综述
Mycobacterium tuberculosis
Genetic heterogeneity
Whole-genome sequencing
Infection
Review
