miR-375在乳腺癌增殖和临床预后相关性中的研究

Correlation between miR-375 and cell proliferation and clinical prognosis in patients of breast cancer

目的:研究miR-375在乳腺癌组织及癌旁的表达特性,探讨其与临床病理因素的关联性,以及在乳腺癌细胞增殖中的作用。方法:选取45例经手术切除的乳腺癌组织标本(实验组)和对应的癌旁组织标本(对照组),通过实时荧光定量PCR法分析目标组织内miR-375的表达情况。结合临床资料(年龄、癌肿直径、腋窝淋巴结阳性总量)和病理因素(组织学分级、HR、Her-2),比较不同临床病理参数与患者miR-375表达差异,分析其中的关系。并过表达miR-375,检测乳腺癌细胞的增殖。结果:①通过RT-qPCR实验表明,乳腺癌组织与其癌旁组织相比miR-375的表达程度减少(P<0.001)。②miR-375的表达差异与患者的癌肿直径、年龄、组织学分级、HR状态、Her-2状态无关联性(P>0.05),与淋巴结转移存在关联性(P<0.05)。③miR-375的过表达可抑制乳腺癌细胞增殖(P<0.05)。结论:miR-375可作为乳腺癌患者预后分析的重要依据及潜在的乳腺癌治疗靶点。

Objective:To observe miR-375 expression in breast cancer and its adjacent tissues,and investigate the correlation between miR-375 and clinicopathological factors in breast cancer tissues as well as the role of this gene in the cell proliferation.Methods:Breast cancer and its corresponding adjacent tissues were obtained from 45 patients undergone surgical resection,divided into experimental group and control group,and detected for the expression of miR-375 using real-time fluorescence quantitative PCR(RT-qPCR).Then the two groups were compared regarding the difference in clinicopathological factors and miR-375 expression based on the clinical data(the patient′s age,cancer size,total positive axillary lymph nodes)and pathological factors(histological grade,expression of HR and Her-2 protein).Finally,miR-375 was overexpressed to observe the proliferation of breast cancer cells.Results:①RT-qPCR showed that miR-375 expression in breast cancer tissues was significantly reduced compared with that in adjacent tissues(P<0.001);②Expression of miR-375 was not correlated with tumor size,patient′s age,histological grade,and expression status of HR and Her-2(P>0.05),yet with lymph node metastasis(P<0.05);③Overexpression of miR-375 suppressed the proliferation of breast cancer cells.Conclusion:miR-375 can serve as an important basis for prognostic analysis and potential target in treating breast cancer.