文冠果花提取物对良性前列腺增生具有抑制作用

Xanthoceras sorbifolium Bunge flower extract inhibits benign prostatic hyperplasia in rats

目的 探究文冠果花提取物对良性前列腺增生的抑制作用及可能的机制。方法 通过MTT法检测文冠果花提取物对良性前列腺增生细胞(BPH-1)增殖能力的影响、Annexin V/PI双染色法检测细胞凋亡、流式细胞仪测定细胞周期、免疫印迹法检测BPH-1细胞Bcl-2/Bax/Caspase3、PI3K/AKT蛋白表达水平。通过皮下注射丙酸睾酮建立大鼠良性前列腺增生(BPH)模型,文冠果花提取物给药28 d后取大鼠前列腺组织,通过HE染色观察组织病理变化,免疫印迹法检测前列腺组织中Bcl-2/Bax/Caspase3、PI3K/AKT的蛋白表达水平。结果 文冠果花提取物在125-1000μg/mL的浓度范围内抑制BPH-1细胞的增殖(P<0.05);在G0/G1期产生周期阻滞,细胞的凋亡率与文冠果花提取物给药浓度正相关;文冠果花提取物处理后的细胞Bcl-2蛋白表达水平明显下调,Bax和Caspase3蛋白表达水平显著提升,PI3K/AKT蛋白磷酸化水平显著下调(P<0.05);在动物实验中,与模型组相比,文冠果花提取物中剂量组和高剂量组p-AKT、Bcl-2表达水平降低,Bax表达水平升高,低剂量组p-AKT表达水平下降(P<0.05)。结论 通过BPH-1细胞和BPH动物模型,验证了文冠果花提取物对良性前列腺增生具有抑制作用。

Objective To assess the inhibitory effect of the extract of Xanthoceras sorbifolium Bunge flower against benign prostatic hyperplasia(BPH) and explore its possible mechanism. Methods MTT assay was used to examine the effect of the extract of Xanthoceras sorbifolium Bunge flower on proliferation of benign prostatic hyperplasia cells(BPH-1), and cell apoptosis and cell cycle changes following the treatment were analyzed using annexin V/PI double staining and flow cytometry. The protein expression levels of Bcl-2, Bax, caspase-3, PI3K and AKT in the treated cells were detected using Western blotting. A rat model of BPH established by subcutaneous injection of testosterone propionate was treated with the flower extract for 28 days,and pathological changes in the prostate tissue were observed with HE staining. The protein expression levels of Bcl-2, Bax,caspase3 and PI3K/AKT in the prostate tissue were detected with Western blotting. Results Within the concentration range of 125-1000 μg/mL, the flower extract of Xanthoceras sorbifolium Bunge significantly inhibited the proliferation of BPH-1 cells and caused obvious cell cycle arrest at G0/G1 phase;the apoptotic rate of the cells was positively correlated with the concentration of the flower extract(P<0.05). Bcl-2, p-PI3K and p-AKT expression levels were significantly down-regulated and Bax and caspase-3 expression levels were significantly increased in the cells after treatment with the flowers extract(P<0.05). In the rat models of BPH, the rats treated with the flowers extract at moderate and high doses showed obviously decreased expressions of p-AKT and Bcl-2 and an increased expression of Bax in the prostate tissue;a significantly lowered p-AKT expression was observed in the prostate tissue of rats receiving the low-dose treatment(P<0.05). Conclusion The flower extract of Xanthoceras sorbifolium Bunge has a inhibitory effect on BPH both in vitro and in rats, suggesting its potential value in the development of medicinal plant preparations for treatment of BPH.