摘要
目的:研究钛种植体载锶纳米表面的生物学效应,及其对骨质疏松大鼠骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)成骨分化的作用。方法:以纯钛(titanium,Ti)片为对照组(control-Ti),用氢氧化钠(NaOH)水热处理纯钛片后获得纳米组(nanometric-Ti, nm-Ti),用Sr(OH)2水热处理纯钛片获得载锶(strontium,Sr)纯钛组(Sr-Ti),将纯钛片先后以NaOH、Sr (OH)_(2)两步法水热处理获得载锶纳米组(nm/Sr-Ti)。通过扫描电镜(scanning electron microscope,SEM)、X射线光电子能谱(X-ray photoelectron spectroscopy,XPS)和X射线衍射(X-ray diffraction,XRD)技术等检测各组表面性能。采用双侧卵巢切除术(ovariectomy,OVX)建立骨质疏松大鼠模型,10周后提取BMSCs,通过CCK-8法和实时定量聚合酶链反应(real-time quantitative polymerase chain reaction,RT-qPCR)评价各组钛表面对正常及骨质疏松大鼠BMSCs成骨能力的影响。结果:与control-Ti组相比,nm-Ti组和nm/Sr-Ti组表面均呈现成簇片状纳米结构,Sr-Ti组和nm/Sr-Ti组表面负载Sr离子。CCK-8结果显示,正常及骨质疏松大鼠BMSCs在各组钛片表面均呈现良好的黏附和增殖能力,其中nm/Sr-Ti组细胞增殖最活跃。RT-qPCR结果显示,nm-Ti组和nm/Sr-Ti组成骨基因OCN、OPN及BMP-2表达随时间推移升高,且与对照组相比,差异显著。在骨质疏松大鼠模型中,nm/SrTi组显著促进了BMSCs早期BMP-2的表达。结论:载锶纳米钛表面有利于BMSCs的黏附、增殖和分化,并可能对骨质疏松大鼠的种植体周围骨整合有促进作用。
Objective:To study the biological effect of strontium-loaded nano-titanium surface and its effects on osteogenic differentiation of bone marrow mesenchymal stem cells(BMSCs)of osteoporotic rats in vitro.Methods:The cleaned and dried titanium sheets were used as the control group(control-Ti).Using a hydrothermal method,the cleaned titanium sheets were treated with NaOH solution to achieve nanometric-Ti group(nm-Ti).And by hydrothermally treating titanium sheets using Sr(OH)_(2) solution,we achieved strontium-loaded titanium group(Sr-Ti).At last,we achieved strontium-loaded nano-titanium group(nm/Sr-Ti)by a two-step hydrothermal treatment of NaOH and Sr(OH)_(2) solution.Then,surface charac teristics of these groups were analyzed by scanning electron microscope(SEM),X-ray photoelectron spectroscopy(XPS)and X-ray diffraction(XRD)measurement.A rat model of osteoporosis was also established by bilateral ovariectomy(OVX)in female rats.After 10 weeks,the BMSCs were extracted and seeded on the surfaces of different titanium sheets.CCK-8 and real-time quantitative polymerase chain reaction(RT-qPCR)were used to evaluate the effects of different titanium sheet surfaces on the osteogenesis of BMSCs of osteoporotic rats.Results:According to the SEM results,the surfaces of nm-Ti and nm/Sr-Ti group compared with that of control-Ti group showed nano-sheet structures,and Sr element was detected on the surfaces of the Sr-Ti group and the nm/Sr-Ti group.The result of CCK-8 showed that the number of BMSCs from normal and osteoporotic rats increased with time on the surface of each titanium sheets,and nm/Sr-Ti group proliferated the most.The result of RT-qPCR showed that the expression levels of OCN,OPN and BMP-2 genes in nm-Ti group and nm/Sr-Ti group significantly increased with time compared to control-Ti group,and the changes in nm/Sr-Ti group were the most significant.Conclusion:Strontium-loaded nano-titanium surface is beneficial to the adhesion,proliferation and differentiation of BMSCs,and has the potential to promote osseointegration around implants in osteoporotic rats.
作者
Shahrzad Hosseinijenab
王静文
苏俭生
张磊
Shahrzad Hosseinijenab;WANG Jingwen;SU Jiansheng;ZHANG Lei(Shanghai Engineering Research Center of Tooth Restoration and Regeneration,Department cf Prosthodontics,School and Hospital of Stomatology,Tongji Unwersity,Shanghai 200072,China)
出处
《口腔颌面外科杂志》
CAS
2022年第5期278-283,共6页
Journal of Oral and Maxillofacial Surgery
基金
国家自然科学基金(81873721)
上海市自然科学基金(18ZR1443000)。
关键词
钛种植体
纳米表面
锶离子
骨质疏松
成骨分化
titanium implant
nano-structure surface
strontium
osteoporosis
osteogenic differentiation