油田采出水微生物菌株的筛选及适应性评价

Screening and Adaptability Evaluation of Microbial Strains in Oilfield Produced Water

微生物处理油田采出水技术已在各油田广泛应用,如何针对地层条件、区块采出水特性筛选适宜的采出水降解菌株需要进一步探讨。文章从长庆油田姬嫄油田某联合站采出水中分离纯化出5种微生物菌株,分别研究温度、酸碱性、盐度等条件对筛选菌株的影响,从而探讨菌株对该区块油藏条件的适应性;结果表明5种菌株能够适应该区块油藏条件,其中A菌株生长繁殖趋势最好;进一步开展5种筛选菌株降解原油性能的实验,结果表明A菌株对石油类的降解率达到55.6%,可作为油田采出水降解菌菌株。

Microbial treatment of oilfield produced water has been widely used in various oilfields. How to screen suitable produced water degradation strains according to formation conditions and block produced water characteristics needs to be further discussed. In this paper, five microbial strains were isolated and purified from the produced water of a block in Dingbian, Changqing Oilfield by plate streaking. Through simulation experiments, the adaptability of the screened strains to the main reservoir conditions, including temperature, acidity and alkalinity, salinity, were studied respectively. The results showed that the five strains could adapt to the reservoir conditions of the block, and the growth and reproduction trend of strain A was the best. In addition, the degradation rate of petroleum by strain A was more than 55.6%,which could be used as a strain of degrading bacteria in oilfield produced water.