盐酸体积分数对蓝莓花色苷提取率及抗氧化性和稳定性的影响

Effects of Volume Fraction of Hydrochloric Acid on Extraction Rate,Antioxidant Activity and Stability of Blueberry Anthocyanins

蓝莓花色苷的高效提取及分离对蓝莓资源的开发和应用具有重要意义。文章采用超声-微波协同辅助技术,以无水乙醇为溶剂提取蓝莓花色苷,探究不同盐酸体积分数对花色苷提取率及其体外抗氧化性的影响,并采用三重四极杆飞行时间精密质谱仪鉴定花色苷组成成分。结果表明:提取花色苷的最佳盐酸体积分数为0.2%,花色苷含量为0.534 mg/g。在一定盐酸体积分数范围内,花色苷的DPPH自由基清除率随盐酸体积分数的增大而提升;低温与酸性条件下花色苷更为稳定。液质结果表明花色苷提取物中至少含有8种成分。研究结果可为超声微波协同技术在花色苷的开发与利用方面提供一定的理论基础和数据支撑。

The efficient extraction and separation of blueberry anthocyanins is of great significance to the development and application of blueberry resources.In this paper,ultrasonic-microwave assisted technology was used to extract blueberry anthocyanins with anhydrous ethanol as solvent,and the effects of different volume fraction of hydrochloric acid on the extraction rate and antioxidant activity of anthocyanins were studied.The components of anthocyanins were identified by quadrupole time-offlight mass spectrometry.The results showed that the optimum volume fraction of hydrochloric acid for anthocyanin extraction was 0.2%,and the extraction content was 0.534 mg/g.In a certain range of volume fraction of hydrochloric acid,the antioxidant activity of anthocyanins can be improved with the increase of volume fraction of hydrochloric acid,and anthocyanins are more stable under low temperature and acidic conditions.The LC-MS results showed that there were at least 8 components in the anthocyanin extract.In summary,the research can provide some theoretical basis and data support for the development and utilization of blueberry anthocyanin resources by ultrasonic microwave cooperative technology.