一种稳定型绿色消毒剂抗菌作用的研究

Study on the antibacterial effect of a stable green disinfectant

目的分析一种稳定型绿色消毒剂的抗菌作用。方法通过54℃加速对比实验测定微酸性氧化电位水有效氯的残存情况,分析确定最优的稳定剂及用量。对优化后的消毒剂通过稳定性加速试验进行理化性能评价,测定其有效氯的含量、pH值和氧化还原电位。按照消毒技术规范要求,采用空气杀毒效果试验、悬液定量杀菌实验观察消毒剂进行空气消毒、大肠埃希菌、副溶血性弧菌和白色念珠菌杀灭效果。按照消毒剂规范要求进行其皮肤刺激性实验。结果添加稳定剂的消毒剂能通过稳定性加速试验,有效氯含量下降率<10%,pH值和ORP无显著变化,贮藏有效期保持1年以上。该消毒剂可以同时杀灭大肠埃希菌、副溶血性弧菌和白色念珠菌3种指标菌。对健康成年家兔皮肤的皮肤刺激指数为0,其刺激强度级别可判为无刺激性。结论该稳定型消毒剂对多种致病菌具有较好的杀菌效果,且具有长期稳定性,属实际无毒物质,对皮肤无刺激性,是一种安全有效的消毒剂。

Objective Based on the quality research,the antibacterial effect of a stable green disinfectant was mainly observed,so as to provide a scientific reference for the popularization and application of the disinfectant.Methods The residual situation of available chlorine in slightly acidic oxidizing potential water was determined by accelerated comparative experiment at 54℃,and the optimal stabilizer and dosage were determined.The physical and chemical properties of the optimized disinfectant were evaluated by stability accelerated test,and its effective chlorine content,pH value and redox potential were measured.According to the requirements of the disinfection technical specifications,the air disinfection effect test and suspension quantitative sterilization test were used to observe the disinfection effects on the air disinfection,E.coli,V.parahemolyticus and C.albicans.The skin irritation experiment was conducted according to the disinfectant specification.Results The disinfectant added with stabilizer passed the stability accelerated test,and the decrease rate of effective chlorine content was less than 10%.There was no obvious change in pH value and ORP.Finally,the shelf life was maintained for more than 1 year.The disinfectant can simultaneously kill the three indicator bacteria:E.coli,V.parahemolyticus and C.albicans.The skin stimulation index of healthy adult rabbit skin was 0,and its stimulation intensity level can be judged as non-irritating.Conclusion The stable disinfectant was proved to have good bactericidal effect on a variety of pathogenic bacteria and long-term stability.It has been confirmed to be actually a non-toxic substance without irritation to the skin.It was an effective,safe and ideal disinfectant.