摘要
CRISPR/Cas9系统是一项简单、高效的基因定点编辑技术,在植物遗传改良及作物良种选育等方面具有重要的应用价值。本研究主要介绍了CRISPR/Cas9的原理及构建方法,论述了近年来CRISPR/Cas9技术在植物基因功能及基因表达调控、植物基因组的定向编辑以及作物分子育种中的应用及研究进展,分析了该基因编辑系统的主要影响因素及优化改进方式,探讨了该系统在应用中的问题及解决途径并对今后发展方向进行了展望,为该技术在植物基因组定点编辑及作物遗传育种等领域研究提供参考。
The CRISPR/Cas9 system is a simple and efficient targeted gene editing technology,which has important application value in plant genetic improvement and crop genetics and breeding.This research mainly introduced the principle and construction method of CRISPR/Cas9,discussed the application and research progress of CRISPR/Cas9 in plant gene function and gene expression regulation,plant genome site-specific editing and crop molecular breeding in recent years.We analyzed the main influencing factors and the optimizing methods of this gene editing technology,as well as problems in its application and the solutions to the problems,and discussed the future development direction of the technology,thus providing reference for the application of CRISPR/Cas9 in plant genome editing and crop genetics and breeding.
作者
权莹
张晓娟
赵辉
孙晓敏
马秀奇
QUAN Ying;ZHANG Xiaojuan;ZHAO Hui;SUN Xiaomin;MA Xiuqi(School of Biological Sciences and Engineering,Shaanxi University of Technology,Hanzhong,Shaanxi 723001;Department of Physical Education,Shaanxi University of Technology,Hanzhong,Shaanxi 723001;Hanzhong Agricultural Science Institute,Hanzhong,Shaanxi 723001)
出处
《中国农学通报》
2022年第26期9-14,共6页
Chinese Agricultural Science Bulletin
基金
陕西省科技厅项目“油菜菌核病抗性相关基因分子标记开发及种质资源创新”(2020NY-068)
陕西理工大学人才启动项目“甘蓝型油菜菌核病抗性基因的筛选鉴定”(SLGRCQD2115)
陕西理工大学2020年大学生创新创业训练计划项目“油菜P5CR基因克隆及其多态性与菌核病抗性相关性研究”(S202010720022)。
