基于高通量测序技术的贵州不同类型细菌型豆豉菌群多样性分析

Community diversity of different bacterial-type of Guizhou Douchi using high throughput sequencing

该研究利用高通量测序技术探究贵州干豆豉、湿豆豉和水豆豉之间的细菌群落结构及其组成差异。通过对测序结果进行分类注释,共得到1098891条有效数据,884个操作分类单元(operational taxonomic units,OTUs),共注释获得5个门类,96个属类。在门水平上,厚壁菌门(Firmicutes)和变形菌门(Proteobacteria)为主要菌门,在3类豆豉中丰度高达99.0%以上;属水平上,干豆豉中主要的细菌类群为芽孢杆菌属(Bacillus)和变形杆菌属(Proteus);湿豆豉丰度最高的为芽孢杆菌属和普罗威斯登菌属(Providencia);水豆豉则主要由芽孢杆菌属和四联球菌属(Tetragenococcus)组成。3类豆豉均以芽孢杆菌属为优势菌属,在干豆豉和湿豆豉中芽孢杆菌属的平均相对丰度为93.0%和74.3%,水豆豉中为42.3%。此外,聚类分析和主成分分析结果表明,干豆豉与湿豆豉细菌组成更为相似,而水豆豉细菌种类更加丰富。该研究拟为贵州豆豉的标准化及其安全管理提供理论依据。

The bacterial community structures and composition differences of three types of Guizhou Douchi,namely dry Douchi,wet Douchi and water Douchi,were investigated and compared by high-throughput sequencing technology.A total of 1098891 valid data and 884 bacterial operational taxonomic units(OTUs)were obtained,and clustered into five bacterial phyla and 96 genera.The dominant bacterial phyla were Firmicutes and Proteobacteria,accounting for more than 99.0%in the all three types of Douchi.Bacillus and Proteus were the major genera in dry Douchi,and the dominant genera of wet Douchi were Bacillus and Providencia.The samples of water Douchi were abundant in Bacillus and Tetragonococcus.Bacillus was the dominant genus in the three types of Douchi,and the relative abundance of Bacillus in dry Douchi,wet Douchi and water Douchi were 93.0%,74.3%and 42.3%,respectively.In addition,the results of cluster analysis and principal component analysis demonstrated that the bacterial communities of dry Douchi and wet Douchi were similar,and the bacterial species of water Douchi were more abundant than others.This study provides a theoretical basis for the standardization and safety management of Guizhou Douchi.