摘要
目的 卵巢癌是妇科常见的恶性肿瘤,miR-507和RAB43在多种肿瘤细胞中异常表达,研究miR-507通过靶向结合RAB43影响卵巢癌细胞恶性生物学行为的分子机制,为卵巢癌的治疗提供新靶点。方法采用qRT-PCR检测miR-507 mRNA和RAB43 mRNA在卵巢癌细胞中的表达水平,Western blot检测RAB43蛋白在卵巢癌细胞中的表达水平,采用细胞转染方法构建miR-507和RAB43转染细胞系,CCK8法检测SKOV-3细胞增殖情况的改变,划痕实验检测SKOV-3细胞侵袭情况的改变,Transwell实验检测SKOV-3细胞侵袭情况的改变。结果卵巢癌细胞中,miR-507显著低表达,过表达miR-507显著降低SKOV-3细胞的增殖,迁移和侵袭能力;沉默miR-507显著促进SKOV-3细胞的增殖、迁移和侵袭能力。RAB43显著高表达,沉默RAB43显著抑制PI3K/AKT信号通路蛋白的表达,显著抑制SKOV-3细胞的增殖、迁移和侵袭行为;过表达RAB43显著促进PI3K/AKT信号通路蛋白的表达,显著促进SKOV-3细胞的增殖、迁移和侵袭行为。RAB43 3’-UTR区存在miR-507的靶向结合位点,两者结合影响卵巢癌细胞的恶性生物学行为。结论 MiR-507靶向结合转录因子RAB43的3’-UTR区调节卵巢癌细胞的恶性生物学行为。
Objective Ovarian cancer is a common malignant tumor in gynecology. MiR-507 and RAB43 are abnormally expressed in a variety of tumor cells. To study the molecular mechanism of miR-507 influencing the malignant biological behavior of ovarian cancer cells through targeted regulation of RAB43, and to provide new targets for the treatment of ovarian cancer. Methods Real-time quantitative PCR experiment was used to detect the expression of miR-507 mRNA and RAB43 mRNA in ovarian cancer cells. Western blot experiment was used to detect the expression of RAB43 protein in ovarian cancer cells, and cell transfection method was used to construct miR-507 and RAB43 transfection cell line. CCK8experiment was used to detect the change of SKOV-3 cell proliferation ability. The scratch experiment was used to detect the change of SKOV-3 cell invasion ability, and the transwell experiment was used to detect the change of SKOV-3 cell invasion ability. Results In ovarian cancer cells, miR-507 was significantly under-expressed. Overexpression of miR-507significantly reduced the proliferation, migration and invasion capabilities of SKOV-3 cells;silencing miR-507 significantly promoted the proliferation, migration and invasion capabilities of SKOV-3 cells. RAB43 was significantly highly expressed.Silencing RAB43 significantly inhibited the expression of PI3K/AKT signaling pathway proteins, and significantly inhibited the proliferation, migration and invasion behavior of SKOV-3 cells;overexpression of RAB43 significantly promoted the expression of PI3K/AKT signaling pathway proteins, and significantly promoted SKOV-3 cell proliferation, migration and invasion behavior. There was a targeted binding site of miR-507 in the 3’-UTR region of RAB43, and the combination of the two affects the malignant biological behavior of ovarian cancer cells. Conclusion MiR-507 inhibited the malignant biological behavior of ovarian cancer cells by targeting the 3’-UTR region of the transcription factor RAB43.
作者
朱宁
邓岳红
曲振东
ZHU Ning;DENG Yue-hong;QU Zheng-dong(Department of Obstetrics and Gynecology,Sanya Central Hospital/The Third People's Hospital of Hainan Province,Sanya 572019,China)
出处
《解剖科学进展》
CAS
2022年第3期281-286,共6页
Progress of Anatomical Sciences