钩藤内生真菌 Talaromyces sp.次级代谢产物研究

Study on secondary metabolites of endophytic fungus Talaromyces sp.from Uncaria rhynchophylla

综合利用多种色谱分离技术从钩藤内生真菌Talaromyces sp.1.88E的发酵产物中分离得到8个化合物,采用HR-ESI-MS、NMR、IR等波谱解析技术将其鉴定为(Z)-6-methoxy-6-oxo-2-propylhex-2-enoic acid(1)、alteric acid(2)、5′-methoxy-6-methyl-biphenyl-3,4,3′-triol(3)、3-O-methylfunicone(4)、alternariol 1′-hydroxy-9-methyl ether(5)、格链孢酚(6)、5-甲氧基格链孢酚(7)、altenuisol(8)。化合物1为新化合物,化合物2的核磁数据为首次报道。对化合物1~8采用脂多糖(LPS)诱导小鼠单核巨噬细胞RAW 264.7生成NO炎症模型测试抗炎活性,均未表现出抗炎活性。对化合物1~8进行α-葡萄糖苷酶抑制活性测试,化合物2、6对α-葡萄糖苷酶表现出弱的抑制效果,其IC_(50)分别为338.5、375.8μmol/L。

Eight compounds were isolated from the extract of endophytic fungus Talaromyces sp.1.88E from Uncaria rhynchophylla(Miq.)Miq.ex Havil.by various chromatographic techniques.The compounds were identified as(Z)-6-methoxy-6-oxo-2-propylhex-2-enoic acid(1),alteric acid(2),5′-methoxy-6-methyl-biphenyl-3,4,3′-triol(3),3-O-methylfunicone(4),alternariol 1′-hydroxy-9-methyl ether(5),alternariol(6),alternariol methyl ether(7),altenuisol(8)by HR-ESI-MS,NMR,IR and other spectroscopic analysis techniques.Among them,compound 1 was a new fatty acid and NMR data of compound 2 was reported for the first time.Compounds 1-8 showed no obvious inhibition of LPS-induced NO production by RAW 264.7 for the anti-inflammatory test.All isolated compounds were evaluated for the inhibitory activities againstα-glucosidase and compounds 2,6 showed weak inhibitory activity with IC_(50) values of 338.5,and 375.8μmol/L,respectively.