人工构建微生物组对制革废水的氨氮去除及微生物组的对比变化分析

Transformation of Ammonia Nitrogen in Tanning Wastewater by Artificial Microbiome and Comparative Analysis of Microbiome Change

为探究复合人工微生物组对制革废水处理体系中微生物群落的影响,在传统的厌氧/好氧(A/O)污水处理工艺处理制革废水的基础上,投加微生物复合菌形成人工微生物组。利用复合人工微生物组强化废水处理,应用高通量测序技术测定各样品中的细菌16S rRNA V3-V4变异区序列,并对测序数据进行生物信息学分析、Alpha多样性分析以及物种组成分析。结果表明,投加微生物组后,化学需氧量(COD)和氨氮的处理效果得到提升,COD的去除率约为82.60%,氨氮的去除率约为99.47%。高通量测序结果表明,人工投加微生物组使得活性污泥中微生物群落丰度以及多样性提高,污染物降解功能菌占比有所提升,陶厄氏菌属(Thauera)成为其最主要的优势菌属。复合人工微生物组的投加对强化制革废水处理系统有一定潜力。

In order to explore the impact of compound artificial microbial group on the microbial community in the tannery wastewater treatment system, based on the traditional anaerobic/aerobic(A/O) wastewater treatment process, the artificial microbial group was formed by adding microbial compound bacteria.The composite artificial microbiome was used to enhance wastewater treatment, and high-throughput sequencing technology was used to determine the sequence of bacterial 16 S rRNA V3-V4 mutation region in each sample.The sequencing data were analyzed by bioinformatics analysis, Alpha diversity analysis and species composition analysis.The results showed that after the addition of microbial group, the treatment effects of COD and ammonia nitrogen were improved.The removal rate of COD was about 82.60%,and the removal rate of ammonia nitrogen was about 99.47%.The results of high-throughput sequencing showed that the abundance and diversity of microbial communities in activated sludge were improved with the addition of microbial groups, and the proportion of pollutant degradation functional bacteria was increased.The genus Thauera became the most dominant one.The addition of composite artificial microbial group has certain potential for strengthening tannery wastewater treatment.