以阿魏酸为底物产香兰素微生物的筛选及发酵条件优化

Screening of vanillin converting microorganism by ferulic acid and optimization of fermentation conditions

目的:获得以阿魏酸为底物产生香兰素的菌株及最佳发酵工艺条件。方法:以高温大曲为样品,筛选能以阿魏酸为底物产香兰素的菌株,进一步利用摇瓶发酵试验对高产菌株进行单因素试验和正交优化,确定发酵产香兰素的最佳工艺。结果:通过分离筛选得到的高产菌株B2-12经16S rDNA鉴定为枯草芽孢杆菌(Bacillus subtilis)。通过单因素和正交试验优化确定菌株B2-12以阿魏酸为底物转化香兰素的最佳条件为装液量44%、pH 10、43℃、摇床转速160 r/min、发酵4 d,发酵液中的香兰素质量浓度达到(34.5±0.16)mg/L。结论:菌株B2-12能以阿魏酸为底物产生香兰素,具有应用潜力。

Objective:The aim of this study was to obtain vanillin converting microorganism by ferulic acid and optimize of fermentation conditions.Methods:The strains that can produce vanillin by ferulic acid which is screened form Daqu of Baijiu.The single factor experiment and orthogonal experiment were applied to optimize the fermentation conditions by shake flask fermentation.Results:The results showed that isolate B2-12 was identified as Bacillus subtilis and the high yield of vanillin was reached 34.5 mg/L under the conditions of liquid loading 44%,pH 10,43℃and shaking speed 160 r/min for 4 days fermentation.Conclusion:The strain B2-12 can produce vanillin by ferulic acid and it has the potential to apply in the production of milk-flavoring substance.