慢性萎缩性胃炎严重程度与miR-32、miR-181、miR-129的相关性分析

Correlation between Severity of Chronic Atrophic Gastritis and miR-32,miR-181 and miR-129

【目的】探讨慢性萎缩性胃炎(CAG)严重程度与微小RNA-32(miR-32)、微小RNA-181(miR-181)、微小RNA-129(miR-129)的相关性。【方法】选取2019年8月至2021年8月商洛市中心医院收治的130例CAG患者(观察组),另选取同期健康体检的130例健康志愿者(对照组)。比较两组miR-32、miR-181、miR-129表达水平,采用受试者工作特征曲线(ROC曲线)分析miR-32、miR-181、miR-129表达水平预测CAG的价值,比较不同病情严重程度CAG患者miR-32、miR-181、miR-129表达水平,采用Spearman系数分析CAG患者miR-32、miR-181、miR-129表达水平与病情严重程度的相关性。【结果】观察组miR-32、miR-181表达水平显著高于对照组,miR-129表达水平显著低于对照组,差异有统计学意义(P<0.05)。ROC曲线分析显示,miR-32、miR-181、miR-129预测CAG的最佳截断值为3.150、0.813、3.288,曲线下面积分别为0.931、0.880、0.904(P<0.05)。轻度、中度、重度患者miR-32、miR-181表达水平逐渐升高,miR-129表达水平逐渐降低,差异有统计学意义(P<0.05)。Spearman相关性分析显示:CAG患者miR-32、miR-181表达水平与患者病情严重程度呈正相关(P<0.05),miR-129表达水平与病情严重程度呈负相关(P<0.05)。【结论】血清miR-32、miR-181、miR-129表达水平与CAG患者病情严重程度相关,可作为临床预测CAG患者病情严重程度的相关指标。

【Objective】To investigate the correlation between the severity of chronic atrophic gastritis(CAG)and miR-32,miR-181 and miR-129.【Methods】A total of 130 CAG patients(observation group)admitted to the Shangluo Central Hospital from August 2019 to August 2021 were selected,and 130 healthy volunteers(control group)who underwent physical examination at the same time were selected.The expression levels of miR-32,miR-181 and miR-129 were compared between the two groups.The ROC curve was used to analyze the expression levels of miR-32,miR-181,and miR-129 to predict the value of CAG and the expression levels of miR-32,miR-181,and miR-129 in CAG patients with different disease severity were compared at the same time.Spearman coefficient was used to analyze the correlation between the expression level of miR-32,miR-181,miR-129 and the severity of the disease in CAG patients.【Results】The expression levels of miR-32 and miR-181 in the observation group were significantly higher than those in the control group,and the expression levels of miR-129 were significantly lower than those in the control group(P<0.05).ROC curve analysis showed that the best cutoff values of miR-32,miR-181 and miR-129 for CAG prediction were 3.150,0.813 and 3.288,and the areas under the curve were 0.931,0.880 and 0.904 respectively(P<0.05).The expression levels of miR-32 and miR-181 gradually increased in mild,moderate and severe patients,and the expression levels of miR-129 gradually decreased,with a statistically significant difference(P<0.05).Spearman correlation analysis showed that the expression level of miR-32 and miR-181 in CAG patients was positively correlated with the severity of the disease(P<0.05),and the expression level of miR-129 was negatively correlated with the severity of the disease(P<0.05).【Conclusion】The expression levels of serum miR-32,miR-181 and miR-129 are related to the severity of the disease in patients with CAG,and can be used as a relevant indicator to predict the severity of the disease in patients with CAG.