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基于高通量测序技术鉴定松辽黑猪与长白猪背最长肌差异基因 被引量:1

Identification of Differential Genes of Longissimus Dorsi Muscle of Songliao Black Pig and Landrace Pig Based on High-throughput Sequencing Technology
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摘要 旨在利用高通量测序技术对松辽黑猪和长白猪背最长肌组织样本进行mRNA测序和差异分析,筛选影响猪肌肉生长、肉质和脂肪沉积的关键基因,从而为猪肉品质研究提供新的参考信息。采集6头松辽黑猪和6头长白猪的背最长肌组织样本,提取其RNA,采用Illumina HiSeq 2500高通量测序技术对mRNA进行测序,并对获得的reads进行比对、注释和差异表达分析,用NOISeq筛选出差异表达基因并进行相关生物学功能富集分析。结果表明,从2个猪种间共筛选出了664个差异表达基因,其中,364个基因在松辽黑猪中高表达,300个基因在长白猪中高表达。通过对差异表达基因进行生物学功能分析,筛选出LPIN1、FADS1、FADS2、PLIN2、PPARGC1A、PRKAG2和ACSL1等基因参与脂类代谢和肌肉发育相关的调控,相关通路为脂肪酸代谢、PPAR信号通路、AMPK信号通路、胰岛素信号通路和脂肪细胞因子信号通路等。 This study aims to use high-throughput sequencing technology to perform mRNA sequencing and differential analysis of longissimus dorsi muscle tissue samples from Songliao black pigs and Landrace pigs,and screen out key genes that affect pig muscle growth,meat quality and fat deposition,so as to provide pork quality research provides new reference information.The longissimus dorsi muscle tissue samples of 6 Songliao black pigs and 6 Landrace pigs were collected,their RNA was extracted,and the mRNA was sequenced by Illumina HiSeq 2500 high-throughput sequencing technology,and the obtained reads were compared,annotated and differentiated.For expression analysis,NOISeq was used to screen out differentially expressed genes and perform enrichment analysis of related biological functions.The results showed that 664 differentially expressed genes were screened from the two pig species,of which 364 genes were highly expressed in Songliao black pigs and 300 genes were highly expressed in Landrace pigs.Through the biological function analysis of differentially expressed genes,LPIN1,FADS1,FADS2,PLIN2,PPARGC1 A,PRKAG2 and ACSL1 were screened to participate in the regulation of lipid metabolism and muscle development.The related pathways were fatty acid metabolism,PPAR signaling pathway,AMPK signaling pathway,insulin signaling pathway and adipocytokine signaling pathway and so on.
作者 侍玉梅 陈少康 邢凯 赵延辉 原佳妮 盛熙晖 齐晓龙 倪和民 郭勇 王楚端 SHI Yumei;CHEN Shaokang;XING Kai;ZHAO Yanhui;YUAN Jiani;SHENG Xihui;QI Xiaolong;NI Hemin;GUO Yong;WANG Chuduan(Animal Science and Technology College,Beijing University of Agriculture,Beijing 102206,China;Beijing Animal Husbandry Station,Beijing 100101,China;College of Animal Science and Technology,China Agricultural University,Beijing 100193,China)
出处 《华北农学报》 CSCD 北大核心 2022年第5期222-229,共8页 Acta Agriculturae Boreali-Sinica
基金 国家重点研发计划(2018YFD0501000) 生猪产业技术体系北京市创新团队(BAIC02-2021) 北京市教委2019年度科技计划一般项目(KM201910020010) 2022年教委分类发展项目。
关键词 松辽黑猪 长白猪 高通量测序 背最长肌 差异基因 Songliao black pig Landrace pig High-throughput sequencing Longissimus dorsi muscle Differential genes
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