钙对盐胁迫下巴西蕉细胞生长和生理特性的影响

Profiling Cell Growth and Physiological Characteristics of Brazil Banana under Salt Stress

Ca^(2+)对植物的耐盐性具有重要作用。悬浮细胞可以提供相对简化的研究模型,适用于基础的生理机制研究。本试验采用NaCl模拟盐胁迫,外源添加CaCl_(2)、EGTA、LaCl_(3)处理巴西蕉(Musa AAA Cavendish var.Brazil)悬浮细胞,研究细胞生长量、抗氧化酶活性、脯氨酸及可溶性糖含量的变化来确定Ca^(2+)对于盐胁迫下香蕉细胞的调节作用。结果表明,在100 mmol/L NaCl胁迫24 h后,细胞生长量显著下降,下降量接近50%。MDA含量、SOD活性、POD活性和可溶性糖含量均显著升高。10 mmol/L CaCl_(2)将盐胁迫下细胞生长量提高7.95%,POD活性和Pro含量也显著升高,SOD活性降低。EGTA和LaCl_(3)处理加重了盐胁迫对细胞生长的抑制作用,导致MDA含量大幅增加,POD活性和Pro含量降低,其中LaCl_(3)处理使可溶性糖的积累显著降低。以上所示,Ca^(2+)与巴西蕉细胞的耐盐密切相关,外源Ca^(2+)可减轻盐胁迫对细胞的影响。

Ca^(2+) plays an important role in the salt tolerance of plants.Suspension cells can provide a relatively simplified model,which is suitable for basic physiological mechanism research.In this experiment,NaCl was used to simulate salt stress.Suspension cells of Brazil banana(Musa AAA Cavendish var.Brazil)were treated with CaCl_(2),EGTA and LaCl_(3),the changes of cell growth,antioxidant enzyme activity,proline and soluble sugar content were studied to determine the regulatory effect of Ca^(2+) on banana cells under salt stress.The results showed that after 24 hours of 100 mmol/L NaCl stress,the cell growth decreased significantly,and the decrease was close to 50%.While MDA content,SOD activity,POD activity and soluble sugar content increased significantly.10 mmol/L CaCl_(2) increased the cell growth by 7.95%under salt stress and significantly increased the POD activity and Pro content,while decreased the SOD activity.EGTA and LaCl_(3) treatment aggravated the inhibitory effect of salt stress on cell growth,resulting in a significant increase in MDA content,a decrease in POD activity and Pro content,and a significant decrease in soluble sugar accumulation in LaCl_(3) treatment.As shown above,Ca^(2+) is closely related to the salt tolerance of Brazil banana cells,and exogenous Ca^(2+) can reduce the effect of salt stress on cells.