复合型人工湿地对污水厂尾水处理效能及微生物群落研究

Evaluation of the Performance and Microbial Communities by a Hybrid Constructed Wetland System for Treating Wastewater Treatment Plant Tail-Water

为优化人工湿地对污水厂尾水处理效能,构建水生植物湿地+潜流湿地复合型人工湿地,考察不同水力负荷下系统对污染物去除效能,并采用高通量测序对潜流湿地填料层微生物群落结构解析。结果表明系统 NH^(+)-N,TN,COD 和 TP 的去除率随着水力负荷提高而下降,低水力负荷有利于增强系统运行效能,在水力负荷 0.18 m^(3)/(m^(2)·d)下系统对 NH^(+)-N,TN,COD 和 TP 的去除率分别为(51.8±5.2)%,(53.2±4.9)%,(57.5±4.7)%和(58.6±4.3)%。其中水生湿地单元对 NH^(+)-N,TN,COD 和 TP 的去除率分别为(26.6±6.5)%,(21.1±6.1)%,(29.8±6.4)%和(21.6±9.4)%。高通量测序表明 Proteobacteria,Bacteroidetes,Acidobacteria 和 Planctomycetes 为主要优势菌门,FAPROTAX 功能预测分析与氮素代谢相关的功能菌群在系统生物脱氮过程中起到关键作用。

In order to optimize the performance of municipal sewage tail-water treatment,a hybrid constructed wetland process combined an aquaticplant constructed wetland and a vertical flow constructed wetland was designed to investigate the effects of different hydraulic loading conditions to treat the tail water.Meanwhile,high-throughput sequencing was used for analyzing the diversity of microbial community in the vertical flow constructed wetland unit.The results showed that the removal efficiencies of NH4^(+)-N,TN,COD and TP decreased with HLR increased.The removal efficiencies of NH4^(+)-N,TN,COD and TP were(51.8±5.2)%,(53.2±4.9)%,(57.5±4.7)%and(58.6±4.3)%,when the hybrid constructed wetland was operated at an HLR of 0.18 m^(3)/(m^(2)·d).The removal efficiencies of NH4^(+)-N,TN,COD and TP in the aquatic-plant constructed wetland unit were(26.6±6.5)%,(21.1±6.1)%,(29.8±6.4)%和(21.6±9.4)%.In addition,the results also indicated that Proteobacteria,Bacteroidetes,Acidobacteria and Plantomycetes were the predominant phylum,Based on the FAPROTAX functional prediction,the results showed that functional microbial community related to nitrogen metabolism plays a key role in the nitrogen removal process.