氧化锆表面接枝绿原酸-壳聚糖复合物促成骨效果

Effect of grafting chlorogenic acid-chitosan complex on zirconia implant surface on osteointegration

目的:评价氧化锆表面接枝生物活性成分——绿原酸(CGA)-壳聚糖(CS)复合物对骨结合促进的效果。方法:将聚乙烯亚胺(PEI)接枝于氧化锆表面,再接枝用CS包裹后的CGA,建立生物活性涂层。采用接触角计、X射线光电子能谱、扫描电镜等对涂层进行表征分析。利用具有高成骨分化潜能的小鼠前成骨细胞系MC3T3-E1评价涂层的促细胞增殖、粘附及成骨诱导作用。结果:PEI及CGA-CS复合物成功接枝于氧化锆植体表面,该涂层增强了氧化锆表面的亲水性,能促进MC3T3-E1细胞的增殖及粘附,促进MC3T3-E1表达骨钙素(Ocn)、Runt相关转录因子2(Runx2)等成骨相关蛋白。结论:氧化锆植体表面PEI涂层接枝CGA-CS复合物具有促进氧化锆表面成骨的作用。

Objective:To evaluate the effect of zirconia surface grafted bioactive component chlorogenic acid(CGA)-chitosan(CS)complex on the promotion of osseointegration.Methods:CS wrap the CGA and graft it onto the surface of zirconia implants with PEI to build a biologically active coating.Using water contact angle measurement,X-ray photoelectron spectroscopy(XPS),scanning electron microscope(SEM),and other experiments to analyze the coating′s characterization.Pre-osteoblastic MC3T3-E1 cells with high osteogenic differentiation potential was used to evaluate the cell proliferation,adhesion promotion and osteoinduction effects of the coating.Results:PEI and CGA-CS complexes were successfully grafted on the surface of zirconia implants.The coating enhanced the hydrophilicity of zirconia surface,promoted the proliferation and adhesion of MC3T3-E1 cells,and promoted the expression of osteogenic related proteins such as Osteocalcin(Ocn)and Runt related transcription factor 2(Runx2).Conclusions:The PEI coating grafted CGA-CS complexes can promote the osteogenesis on the surface of zirconia.