红芽芋多糖提取工艺优化及其抗氧化活性的研究

Optimum Extraction Process for the Polysaccharides from Red Bud Taro(Colocasia esculenta(L.))Schott and Its Antioxidative Activity in Vitro

【目的】旨在探究料液比、水浴温度及水浴时长对红芽芋多糖得率的影响,进而探究多糖的抗氧化活性。【方法】在单因素基础上,采用 L_(9)(3~4)正交设计优化多糖提取工艺,以红芽芋多糖得率为测定指标,评价其提取效果。以清除过氧化氢、ABTS^(+)自由基、DPPH 自由基、羟自由基(·OH)、超氧阴离子自由基(·O_(2)^(-))能力及总还原力作为研究红芽芋多糖的抗氧化活性相关指标。【结果】红芽芋多糖最佳提取工艺为料液比 1:10 g/mL,水浴提取温度为 90 ℃,水浴提取时长为 4.5 h,该条件下红芽芋多糖平均得率为(6.34±0.20)%;红芽芋多糖清除过氧化氢能力较强,其清除能力与 L-抗坏血酸(VC)相当,红芽芋多糖 ABTS^(+)自由基、DPPH 自由基和·OH 的 IC_(50)分别为 3.97,4.46,9.52 mg/mL,但红芽芋多糖的总还原力不强,对·O_(2)^(-)清除能力较弱。【结论】红芽芋多糖提取工艺优化合理,可用于红芽芋多糖的提取;红芽芋多糖有一定的抗氧化能力,且对不同自由基清除能力存在差异。

[Objective]This study aims to investigate the effects of solid-liquid ratio,water bath tempera-ture and water bath duration on the yield of polysaccharides from red bud taro and its antioxidant activity.[Methods]Based on single factor,L_(9)(3~4)orthogonal design was used to optimize the polysaccharides extractionprocess,and the extraction rate of red bud taro polysaccharides was evaluated as the measurement.The hydro-gen peroxide,ABTS^(+)free radicals,DPPH free radicals,hydroxyl radica(l·OH),superoxide anion radica(l·O_(2)^(-))scavenging ability and the total reducing power were determined to study the antioxidant activity of red bud taropolysaccharides[.Results]The optimal extraction condition of red bud taro polysaccharides were as follows:sol-id-liquid ratio was 1∶10 g/mL,water bath extraction temperature was 90 ℃,water bath extraction time was 4.5 h.Under the optimized condition,the yield of polysaccharides from red bud taro was(6.34±0.20)%.Red bud taropolysaccharides have a strong ability to scavenge hydrogen peroxide,which is comparable with that of L-ascorbicacid(VC).The IC_(50) of polysaccharides against ABTS^(+) free radicals,DPPH free radicals,and·OH was 3.97,4.46,9.52 mg/mL,respectively.However,its total reducing power and the scavenging ability of superoxide anion radi-cals were weak[.Conclusion]The optimized condition in this study can be used for the extraction of red bud ta-ro polysaccharides.Red bud taro polysaccharides have certain antioxidant ability,and its scavenging abilities fordifferent free radicals are different.