基于高通量技术对比分析南洲大曲与茅台大曲的细菌多样性

Comparative Analysis of Bacterial Diversity between Nanzhou Daqu and Maotai Daqu Based on High-Throughput Sequencing

采用高通量测序技术研究南洲大曲和茅台大曲的细菌群落结构及多样性,对比分析两个地区高温大曲的细菌多样性差异。结果表明:细菌门水平,两种大曲均以Firmicutes(厚壁菌门)、Proteobacteria(变形菌门)、Actinobacteria(放线菌门)、Bacteroidetes(拟杆菌门)以及Cyanobacteria(蓝藻菌门)5类为优势菌门;细菌属水平,两种大曲在细菌属水平微生物组成相似但组成比例存在较大差异,南洲大曲的优势菌属为Bacillus(芽孢杆菌属)、Pediococcus(片球菌属)、Weissella(魏斯氏菌属)、Serratia(沙雷氏菌属)等,茅台大曲优势菌属以Bacillus(芽孢杆菌属)、Lactococcus(乳球菌属)、Saccharopolyspora(糖多孢菌属)、Kroppenstedtia(克罗彭斯特菌属)为主。通过对比分析南洲大曲与茅台大曲之间的细菌群落结构和多样性差异,可为后续酱香型大曲工艺的改良以及产品质量的提高提供有益指导。

The bacterial community structure and diversity of Nanzhou Daqu and Maotai Daqu were studied by high-throughput sequencing, and the differences of bacterial diversity between high-temperature Daqu from different areas were compared. The results showed that Firmicutes, Proteobacteria, Actinobacteria, Bacteroidetes and Cyanobacteria were the dominant phyla in the two kinds of Daqu. At the genus level, the bacterial composition of the two kinds of Daqu was similar, but the proportion was quite different. The dominant genera of Nanzhou Daqu were Bacillus, Pediococcus, Weissella, Serratia, etc. The dominant genera of Maotai Daqu were Bacillus, Lactococcus, Saccharopolyspora and Kroppenstedtia. By comparing the differences of bacterial community structure and diversity between Nanzhou Daqu and Maotai Daqu, we have provided useful guidance for the improvement of Jiangxiang Daqu production technology and product quality.