摘要
用组织块分离法和科赫式法则分离鉴定薏苡叶斑病病原菌,并用平板对峙法筛选抑制薏苡叶斑病病原菌生长的生防细菌。结果表明:分离得到的病原菌菌株Y2能够引起薏苡植株与田间病害一致的症状;通过ITS序列扩增,将Y2鉴定为弯孢霉(Curvularia coicis)。通过平板对峙法筛选到1株对Y2有抑制作用的内生细菌菌株L47,L47对薏苡叶斑病病原菌的抑制率为73.82%;经过16S rDNA和gyrA序列分析,将L47鉴定为枯草芽孢杆菌(Bacillus subtilis)。
The Curvularia coicis was isolated and identified by tissue block separation and Koch’s postulares, and the biocontrol bacteria inhibiting the growth of Curvularia coicis were screened by plate confrontation method. The results showed that the isolated Y2 could cause the same symptoms as the field disease. The Y2 was identified as Curvularia coicis by ITS sequence amplification. A strain of endophytic bacteria L47 with inhibitory effect on Y2 was screened by plate confrontation method. The inhibitory rate of the strain against Curvularia coicis was 73.82%. After 16S rDNA and gyrA sequence analysis, the bacterium was Bacillus subtilis.
作者
普凤雅
何开潇
叶建萍
和庆康
谷书杰
何永宏
杨志清
PU Feng-ya;HE Kai-xiao;YE Jian-ping;HE Qing-kang;GU Shu-jie;HE Yong-hong;YANG Zhi-qing(College of Agronomy and biotechnology,Yunnan Agricultural University,Kunming 650201,China;Key Laboratory of Medicinal Plant Biology,Yunnan Agricultural University,Kunming 650201,China;National and Local Joint Engineering Research Center on Germplasm Innovation and Utilization of Chinese Medicinal Materials in Southwestern China,Yunnan Agricultural University,Kunming 650201,China)
出处
《江西农业学报》
CAS
2022年第8期103-109,共7页
Acta Agriculturae Jiangxi
基金
云南省大学生创新创业训练项目“薏苡病原菌分离纯化及致病性检测与鉴定”(SJ2021001)
云南省科技计划项目“云南薏苡规范化种植关键技术研究与示范”(2014RE002)。
关键词
薏苡叶斑病
病原菌
内生细菌
筛选
生物防治
Curvularia coicis
Pathogen
Endophytic bacteria
Screening
Biological control
