枳实白术配伍防治术后肠梗阻的网络药理学研究

A Network Pharmacological Study on the Prevention and Treatment of Postoperative Ileus with Citrus-Atractylodes Compatibility

目的探讨枳实白术配伍防治术后肠梗阻(postoperative ileus,POI)的潜在分子机制。方法通过TCMSP、Uniprot获取药物有效成分及靶点,经OMMI,Genecards,Gene Map,Drugbank,TTD,Disgenet数据库获取POI靶点。采用相关软件构建“药物-成分-靶点”网络、蛋白互作(PPI)网络、蛋白功能团、GO和KEGG通路富集分析。结果获得29个活性成分,CALM1、ICAM1、TIMP1、MMP9、PPARg,CASP3、AKT、IL10、TNF、IL6等10个关键蛋白及2个核心蛋白功能团。KEGG分析(P<0.01)主要涉及IL-17、TNF、HIF-1、PI3K-Akt等信号通路。结论枳实白术配伍可在抗炎、调节免疫、抗粘连、调节神经功能、促胃肠动力等方面协同作用于POI防治,通过钙信号通路发挥抗粘连作用或为防治POI的新思路。

Objective:To study the potential molecular mechanism of the combination of Citrus-Atractylodes compatibility in the prevention and treatment of postoperative ileus(POI).Methods:The active ingredients and targets of drugs were obtained through TCMSP and Uniprot,and the POI targets were obtained through OMMI,Genecards,Gene Map,Drugbank,TTD,and Disgenet databases.Relevant software was used to construct"drug-component-target"network,protein interaction(PPI)network,protein functional group,GO and KEGG pathway enrichment analysis.Results:A total of 29 active ingredients were obtained,including 10 key proteins(CALM1,ICAM1,TIMP1,MMP9,PPARg,CASP3,AKT,IL10,TNF,and IL6)and 2 core protein functional groups.KEGG analysis(P<0.01)mainly involved in IL-17,TNF,HIF-1 and PI3K-Akt signaling pathways.Conclusion:The Citrus-Atractylodes compatibility can synergize the prevention and treatment of POI patients in the aspects of anti-inflammation,regulation of immunity,anti-adhesion,regulation of nerve function,and promotion of gastrointestinal motility and play a role in anti-adhesion effect through calcium signaling pathway or a new idea for the prevention and treatment of POI.