振腹环揉法对PCPA失眠大鼠下丘脑、血清、小肠中SP含量的影响

Effects of Abdomen-vibrating and Round Kneading Method on the SP Content in the Hypothalamus,Serum and Small Intestine of PCPA Insomnia Rats

目的通过检测PCPA失眠大鼠下丘脑、血清、小肠中的SP的浓度,结合PCPA失眠大鼠症状改善的变化,探讨SP在失眠的发生发展过程中之间的变化和作用,阐述振腹环揉推拿法治疗失眠对SP含量的影响。方法选取48只SD大鼠,雌雄各半,随机分为4组,每组12只,空白组不造模不治疗,模型组PCPA法造模后不干预;推拿组PCPA法造模通过振腹环揉推拿干预,药物组PCPA法造模后给予归脾丸灌胃。分别在治疗5 d、10 d各取6只大鼠取材,采用ELISA法检测PCPA失眠大鼠下丘脑、血清、小肠中SP浓度,并观察PCPA失眠大鼠症状改善情况。结果下丘脑SP含量第5 d与第10 d比较模型组、推拿组、药物组血清SP含量均升高(P<0.05),治疗第10 d后,推拿组和药物组与模型组比较,SP含量上升更为显著(P<0.01);大鼠血清SP含量第5 d与第10 d比较,模型组、推拿组、药物组血清含量均显著增高(P<0.01),治疗第10 d后,推拿组和药物组与模型组比较,SP含量上升更为显著,且有显著的统计学意义(P<0.01);PCPA失眠大鼠小肠SP含量第5 d和第10 d比较,模型组、推拿组、药物组小肠SP含量增高(P<0.05),治疗第10 d后,推拿组SP含量上升更为显著(P<0.01)。结论振腹环揉法能够通过调高PCPA失眠大鼠模型下丘脑、血清、小肠内SP浓度,尤其对小肠内SP含量水平的提高效果效果优于药物组,具有改善PCPA失眠大鼠模型失眠症状的作用。

Objective:To explore the changes and roles of SP in the occurrence and development of insomnia and to explain the abdomen-vibrating and round kneading method on SP content in the treatment of insomnia by detecting the concentration of SP in the hypothalamus,serum and small intestine of PCPA insomnia rats and integrating with the changes in symptom improvement of PCPA insomnia rats.Methods:48 SD rats,half male and half male,were randomly divided into 4 groups,12 rats per group.The blank group did not model and did not receive treatment,and the model group did not intervene after modeling by PCPA.The massage group was intervened by abdomen-vibrating and round kneading after modeling by PCPA and the drug group was given Guipi Pill by gavage after modeling by PCPA.On the 5th and 10th day of the treatment,6 rats were collected,and the SP concentrations in the hypothalamus,serum and small intestine of the PCPA insomnia rats were detected by ELISA,and the symptom improvement of the PCPA insomnia rats was observed.Results:Compared among the serum SP levels of the model group,the massage group and the drug group,the SP content of hypothalamus on the 5th day and the 10th day were all increased(P<0.05).After the 10th day of treatment,compared with that of the model group,the SP content of the massage group and the drug group\increased more significantly(P<0.01).Compared with that of the 5th day and the 10th day,the serum SP content of the rats in the model group,massage group and drug group increased significantly(P<0.01).After the 10th day of treatment,compared with that of the model group,the SP content of the massage group and the drug group increased more significantly(P<0.01).Compared with the SP content in the small intestine of the PCPA insomnia rats on the 5th day and the 10th day,the SP content in the model group,the massage group and the drug group were increased more significantly(P<0.01).Conclusion:The abdomen-vibrating and round kneading method can increase the concentration of SP in the hypothalamus,serum and small intestine of PCPA insomnia rat model,especially improve the level of SP in the small intestine of the drug group more effectively,and can improve the insomnia symptoms of PCPA insomnia rat model.