核桃细菌性黑斑病病原分子鉴定及室内药剂筛选

Identification of the Pathogen of Walnut Blight and Bactericide Screening in Laboratory

为确定云南地区核桃细菌性黑斑病的病原菌并筛选防治核桃细菌性黑斑病的有效药剂,采用分子生物学方法鉴定病原菌,牛津杯法测试8种药剂对黑斑病病原菌的抗菌活性,测定8种药剂对黄单胞杆菌和成团泛菌,2种病原菌的室内毒力。结果表明:对2种病原菌均有抑菌活性的是3%噻霉酮、72%农用链霉素和16%苯甲·中生,其中3%噻霉酮和72%农用链霉素对成团泛菌和黄单胞杆菌的EC50分别为3.89、9.97 mg/mL和4.93、14.94 mg/mL,有着较强的抑制作用。

In order to determine the pathogen of walnut blight in Yunnan area and to screen the effective agent for the control of walnut blight.Using molecular biology method to identify pathogenic bacteria and Oxford Cup method to test the antibacterial activity of 8 agents against pathogenic bacteria of walnut blight,and to determine the indoor virulence of 8 agents against Xanthomonas arboricola and Pantoea agglomerans.The results showed that 3%thiamethoxone,72%agricultural streptomycin and 16%benzoate were the most effective antimicrobial agents against both pathogens.Among them,3%thiamethoxone and 72%agricultural streptomycin had strong bacteriostatic effect on both X.arboricola and P.agglomeras.The EC50 values of this 2 antiseptic to P.agglomerans were 3.89–9.97 mg/mL,respectively,and those to X.arboricola were 4.93–14.94 mg/mL.