原花青素对体外培养绵羊卵泡颗粒细胞增殖的影响

Effects of Proanthocyanidin on the Proliferation of Sheep Follicular Granulosa Cells in vitro

旨在研究原花青素对体外培养绵羊卵泡颗粒细胞增殖的影响。不同浓度梯度(20、30、40、50、60、70和80 μg/mL)原花青素的完全培养基中培养绵羊卵泡颗粒细胞,培养时间分别为24、48和72 h,通过MTT法测定颗粒细胞的增殖率;通过RT-qPCR法检测细胞周期相关基因p21、p27和细胞凋亡相关基因caspase-8的表达。结果表明,培养48 h,在原花青素浓度为50 μg/mL时,颗粒细胞的增殖率最高,细胞周期相关基因p21、p27和细胞凋亡相关基因caspase-8这3个基因的表达量均显著降低(P<0.01)。综上所述,原花青素对体外培养绵羊卵泡颗粒细胞有一定的促进增殖效应,并且在相关基因mRNA水平上得到验证。

The aim of this study is to investigate the effects of proanthocyanidin(PC)on the proliferation of sheep follicular granulosa cells in vitro.Sheep follicular granulosa cells(GCs)were cultured in complete medium with different concentrations of PC(20,30,40,50,60,70 and 80 μg/mL)for 24,48 and 72 h,respectively.The proliferation rate of GCs was determined by MTT method.And the mRNA abundances of cell cycle related genes p21,p27 and cell apoptosis-related gene caspase-8 were detected by RT-qPCR.The results showed that the proliferation rate of GCs was the highest when the concentration of PC was 50 μg/mL when cultured for 48 h in vitro.And the relative mRNA abundances of three genes(p21,p27 and caspase-8)significantly decreased,respectively(P<0.01).In sum,PC could promote the proliferation of sheep follicular GCs in vitro,and which was verified at relevant mRNA level.