HPLC法测定舒肝和胃丸主药成分的含量

Determination of Main Components in Shugan Hewei Pills by HPLC

目的:应用高效液相法测定舒肝和胃丸主药成分的含量。方法:采用高效液相色谱仪对舒肝和胃丸中主药成分中芍药苷进行定量测定,以SOD柱为色谱柱,以0.5%磷酸水(用三乙胺调节pH值为3.5):乙腈=86:14为流动相,检测波长为220 nm,进样量:20μl,柱温:25℃,流速:1.0 ml/min。结果:芍药苷在1.011~10.144μg/ml的范围内呈现较好线性关系r=0.992,稳定性RSD=0.13%,平均回收率为99.81%,RSD=0.89%(n=4),平均含量为0.726 mg/粒。结论:本研究中所采用的色谱分离条件可以使芍药苷与其他成分进行完全分离,可用于舒肝和胃丸中芍药苷的含量分析。该方法简单、快速、有效,能为其他中药制剂中芍药苷成分分离提供参考。

Objective:To explore the high-efficiency liquid phase method to determine the content of the main drug composition of Shugan Hewei pills.Methods:Paeoniflorin in Shugan Hewei pills was quantitatively determined by HPLC.SOD column was used as the chromatographic column,0.5%phosphoric acid water(adjusted to pH 3.5 with triethylamine):acetonitrile=86:14 as the mobile phase,the detection wavelength was 220 nm,the injection volume was 20μL,the column temperature was 25℃,and the flow rate was 1.0 ml/min.Results:Paeoniflorin showed a good linear relationship in the range of 1.011–10.144μg/ml(r=0.992).The stability RSD was 0.13%,the average recovery was 99.81%,RSD was 0.89%(n=4),and the average content was 0.726 mg/pellet.Conclusion:The chromatographic separation conditions used in this paper can completely separate paeoniflorin from other components,and can be used for the content analysis of paeoniflorin in Shugan Hewei pills.The method is simple,rapid and effective,and can provide reference for the separation of paeoniflorin in other traditional Chinese medicine preparations.