茶黄素-3’-没食子酸酯-壳聚糖纳米乳液的制备及其性能分析

The Preparation and characteristic analysis of theaflavin-3’-gallate-chitosan nanoemulsion

纳米乳液系统实现了生物活性分子的高效运输、传递,解决了营养物质不稳定、易氧化、生物利用率低等问题。本实验以三聚磷酸钠(Sodium Triphosphate,TPP)作为交联剂,采用离子交联法制备茶黄素-3’-没食子酸酯-壳聚糖(Theaflavin-3’-gallate-Chitosan,TF2B-CS)纳米乳液,所制得乳液粒径为(241.31±6.92)nm,Zeta电位为(13.36±1.03)mV,包埋率为80.34%。通过体外模拟消化研究TF2B-CS纳米乳液的消化规律,对其进行粒径、Zeta电位和微观结构检测,并考察TF2B在小肠消化阶段的生物利用率。在体外模拟消化过程中,胃阶段粒径显著高于原始乳液,各阶段Zeta电位均小于原始乳液;经过口腔-胃-小肠三阶段体外模拟消化后的TF2B生物利用率为9.71%。研究通过制备纳米乳液作为递送TF2B的良好体系,有效地发挥TF2B的生物活性,为进一步优化纳米乳液的制备工艺及其应用提供理论依据。

The nanoemulsion system facilitates efficient transportation and delivery of biologically active molecules,and can solve problems of nutrients unstable,easy oxidation and low bioavailability rates.In this experiment,ionic crosslinking method was used in preparation of theaflavin-3’-gallate-chitosan(TF2B-CS)nanoemulsion using sodium triphosphate(TPP)as the crosslinking agent.The particle size of prepared emulsion was(241.31±6.92)nm,Zeta potential was(13.36±1.03)mV,and encapsulation rate was 80.34%.The digestion law of TF2B-CS nanoemulsion was studied by in vitro simulated digestion.Nanoemulsion particle size,Zeta potential and microstructure were tested,and bioavailability rate of TF2B in the digestion stage of small intestine was investigated.In the process of simulated digestion in vitro,particle size of the gastric stage was significantly higher than that of the original emulsion,and zeta potential of each stage was smaller than that of the original emulsion;the bioavailability rate of TF2B after three-stage of oral-gastric-small intestine simulated digestion in vitro was 9.71%.The study prepared a nanoemulsion system for delivering TF2B and effectively utilized bioactivities of TF2B.It provides a theoretical basis for further optimizing the preparation process of nanoemulsion.