油菜素内酯对低温胁迫下冬小麦wrab17基因表达的影响

Effect of Brassinolide on wrab17 Gene Expression in Winter Wheat under Low Temperature Stress

为了探索油菜素内酯(BR)对低温胁迫下 wrab17基因表达模式的影响,以寒地冬小麦品种东农冬麦1号(Dn1)为试验材料,利用qRT-PCR技术克隆得到 wrab17基因的cDNA序列,利用生物信息学方法分析该序列的特征,采用qRT-PCR技术检测低温(5℃、0℃、-10℃、-25℃)胁迫下该基因在冬小麦叶片和分蘖节中的表达情况以及BR对 wrab17基因表达量的影响。结果表明, wrab17基因的编码区序列长度为735 bp,包含500 bp的完整开放阅读框,可编码167个氨基酸,属于稳定蛋白质,该蛋白质无跨膜区和信号肽。qRT-PCR分析表明,低温胁迫下对照组和BR处理组的Dn1叶片和分蘖节中, wrab17基因的相对表达量分别在-10℃和-25℃时最高,且BR处理组 wrab17基因的相对表达量均高于对照组,推测BR可通过提高 wrab17基因的表达量来增强冬小麦的抗寒能力。

In order to explore the effect of brassinolide(BR) on the expression of wrab17 gene under low temperature, the cDNA sequence of wrab17 gene was cloned by qRT-PCR using Dongnongdongmai 1(Dn1) as the experimental material.The characteristics of the sequence were analyzed by bioinformatics methods. qRT-PCR method was used to characterize the expression pattern of wrab17 gene in winter wheat leaves and tillering nodes under four temperatures of 5 ℃,0 ℃,-10 ℃and-25 ℃ and the effect of BR on the expression of wrab17 gene.The results showed that the cDNA sequence of wrab17 gene is 735 bp in length, which contains a complete open reading frame of 500 bp, encoding 167 amino acids, and is classified as stable protein, which has no transmembrane region and signal peptide.The results of qRT-PCR analysis showed that under low temperature stress, the expression level of wrab17 genes of wheat leaves and tiller nodes in the control group and BR treatment group were the highest at-10 ℃ and-25 ℃,respectively.The expression level of wrab17 gene in the BR treatment groups were generally higher than that in the control groups, indicating that BR can increase the cold tolerance of winter wheat by increasing the expression of wrab17 gene.