经典血清分型法与分子分型法对肺炎链球菌血清分型的检测结果比较

Comparison of serotyping results of Streptococcus pneumoniae by classical serotyping and molecular typing

目的比较血清分型法(经典荚膜肿胀法)与分子分型法[多重聚合酶链式反应(PCR)法]对肺炎链球菌(SP)的检测效果,旨在为临床提供快捷、可靠的血清分型方法。方法选取50株肺炎链球菌为研究对象,分别采用经典荚膜肿胀法和多重PCR法进行血清分型检测。比较两种方法的血清分型率。结果多重PCR法血清分型率为76.0%,高于经典荚膜肿胀法的44.0%,差异具有统计学意义(P<0.05)。结论与血清分型技术经典荚膜肿胀法比较,分子分型法多重PCR法可快捷、可靠、敏感地进行肺炎链球菌血清分型,可以广泛应用。

Objective To compare the serotyping results of Streptococcus pneumoniae(SP)by classical serotyping(classical capsular swelling)and molecular typing[multiplex polymerase chain reaction(PCR)],aiming to provide a fast and reliable serotyping method for clinical practice.Methods 50 strains of Streptococcus pneumoniae were selected as the research objects,and serotypes were detected by classical capsular swelling and multiplex PCR respectively.The serotyping detection rate and coincidence rate of the two methods were compared.Results The serotyping rate of multiplex PCR was 74.0%,which was higher than 44.0%of classical capsular swelling,and the difference was statistically significant(P<0.05).Conclusion Compared with classical capsular swelling of serological typing,the molecular typing method of multiplex PCR can perform Streptococcus pneumoniae serotyping quickly,reliably and sensitively,and can be widely used.