烯效唑浸种对干旱胁迫下燕麦种子萌发及幼苗抗旱生理特性的影响

Soaking Oat Seeds with Uniconazole to Improve Their Germination and Resistance of Seedlings to Drought Stress

【目的】探究不同质量浓度烯效唑浸种对2个燕麦品种种子萌发、幼苗生长和抗旱生理特性的影响。【方法】采用二因素随机试验,2个燕麦品种分别为0117-119(V1)和YY11-18(V2),烯效唑设4个质量浓度,分别为0mg/L(U1)、15mg/L(U2)、30mg/L(U3)和45mg/L(U4)。【结果】V1U1处理的发芽势为52.00%,显著高于其他处理(p<0.05);V1U2处理的发芽率为72.67%,显著高于其他处理(p<0.05)。V2U4处理的发芽势和发芽率均最低,分别为18%和36%。苗高、最大根长及冠层干质量均表现为V1U1处理最高,V2U4处理最低。V1U2处理的侧根数、根干质量及根冠比均最高,分别为6.30个、4.33 mg和0.34,均显著高于其他各处理(p<0.05)。V1U2处理的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性、根系活力和脯氨酸量均最高,分别达到150.22 U/g、34.83 U/g、387.35 U/g、239.43μg/(g·h)和298.88μg/g,均显著高于其他各处理(p<0.05),但是,V1U2处理的丙二醛量则显著低于其他处理(p<0.05),为7.28 nmol/g。V1U4处理的叶绿素a、叶绿素b和总叶绿素量最高,分别为1.51、0.58 mg/g和2.12 mg/g,均显著高于其他处理(p<0.05)。因此,适宜质量浓度烯效唑处理可促进燕麦根系的生长发育,并通过调控燕麦植物体内保护酶的活性,有效清除活性氧来降低干旱对植株引起的伤害,促进有机物质的合成,提高燕麦的抗旱能力。【结论】综上可知,燕麦品种0117-119的抗旱能力优于品种YY11-18,15 mg/L烯效唑质量浓度浸种最好,品种0117-119和15 mg/L烯效唑的处理组合较优。

【Objective】Promoting technologies are often used to improve seed germination. In this paper, we study the efficacy of soaking oat seeds with uniconazole in improving their germination and resistance of the seedlings against drought stress. 【Method】We compare two oat varieties, 0117-119(V1) and YY11-18(V2). Seeds of each variety are soaked by uniconazole at concentrations 0 mg/L(U1), 15 mg/L(U2), 30 mg/L(U3) and 45 mg/L(U4),respectively. The germination pattern and the seedling traits in each treatment is measured. 【Result】The highest germination potential and germination rate of V1 are 52.0% and 72.67%(p<0.05), achieved under U1 and U2,respectively. The least germination potential and germination rate are V2 under U4, being 18% and 36%,respectively. V1 under U1 achieves the highest seedling shoot length, maximum root length and crown root dry weight, while V2 under U4 gives the least. The number of lateral roots, root dry weight and root-crown ratio of V1under U2 are significantly higher than those in other treatments(p<0.05), being 6.30, 4.33 mg and 0.34, respectively.V1 under U2 gives the best SOD, POD, CAT, root vigor and proline contents, being 150.22 U/g, 34.83 U/g, 387.35U/g, 239.43 μg/(g·h) and 298.88 μg/g, respectively, but the lowest malondialdehyde content at 7.28 nmol/g. The contents of chlorophyll a and b, as well as the total chlorophyll in V1 under U4 are significantly higher than those in other treatments(p<0.05), being 1.51 mg/g, 0.58 mg/g and 2.12 mg/g, respectively. V1 is more drought resistant than V2. Soaking the seeds by uniconazole at concentration of 15 mg/L is optimal for both varieties, and V1 under U2works the best. 【Conclusion】Soaking oat seeds with uniconazole can effectively alleviate the damage caused by drought, thereby improving their germination rate. Our results can help design oat seed drill in semi-arid areas that are facing drought stress in northern China.