HPLC法同时测定防己中木兰碱、轮环藤酚碱和荷包牡丹碱的含量

Simultaneous determination of magnoflorine,cyclanoline and dicentrine in Stephania tetrandra S.Moore by HPLC

目的探索对防己中木兰碱、轮环藤酚碱和荷包牡丹碱的HPLC测定方法。方法色谱柱为Kromasil C_(18)柱,流动相:以乙腈(C_(2)H_(3)N)-0.4%磷酸(H_(3)PO_(4))溶液为混合流动相,采用梯度洗脱,柱温35℃,流速1.0 ml/min,检测波长280 nm。结果木兰碱、轮环藤酚碱和荷包牡丹碱分别在4.80~24.00μg/ml、21.21~106.07μg/ml和8.85~44.27μg/ml范围内线性关系良好,平均回收率分别为98.97%(RSD为1.01%)、100.16%(RSD为1.78%)和99.23%(RSD为1.97%)。结论本含量测定方法分离度好、重复性优、准确度高,可以作为防己药材质量控制的一种有效方法。

Objective To establish a method for Simultaneous determination of magnoflorine,cyclanoline and dicentrine in Stephania tetrandra S.Moore by HPLC.Methods The Kromasil C_(18) column was used with a mobile phase of acetonitrile-0.4% phosphoric acid gradient elution.The column temperature was 35℃.The flow rate was 1.0 ml/min.The detection wavelength was 280 nm.Results The linear range of magnoflorine,cyclanoline and dicentrine were 4.80~24.00μg/ml、21.21~106.07μg/ml和8.85~44.27μg/ml.The average recoveries were 98.97%(RSD=1.01%)、100.16%(RSD=1.78%)and 99.23%(RSD=1.97%),respectively.Conclusion The method has good separation,repeatability and high accuracy.It provides a reference for the quality control of Stephania tetrandra S.Moore.