HIV-1 DNA的检测及临床应用

Detection and clinical application of HIV-1 DNA

HIV-1储存库的持续存在是治愈HIV的主要障碍,在临床研究中,需要可靠的生物标志物对其进行标记。HIV-1 DNA在HIV-1储存库中可被持续检测到,在HIV-1感染诊断、预测病毒反弹和监测治疗效果等方面具有重要应用价值。PCR的检测技术是临床上常用的HIV-1 DNA检测方法,随着技术的不断创新与进步,可更准确地通过定性或定量检测感染细胞中总的、整合的和未整合的HIV-1 DNA。感染细胞中不同形式的HIV-1 DNA作为生物标志物在HIV感染监测和艾滋病治疗相关研究中报道日益增多。本文对感染细胞中HIV-1 DNA的检测方法及其作为生物标志物的临床应用进展进行综述。

The persistence of the HIV-1 reservoir is still the main obstacle to the cure of HIV.In clinical research,reliable biomarkers are needed to label it.HIV-1 DNA can be continuously detected in the HIV-1 reservoir.It has significant application value in diagnosing HIV-1 infection,the timing of antiretroviral therapy,the prediction of virus rebound,and monitoring treatment effects.The detection technology based on polymerase chain reaction(PCR)is the most commonly used HIV-1 DNA detection method in clinical practice.The continuous innovation and advancement of technology can accurately detect the total,integrated,and unintegrated HIV-1 DNA in infected cells using qualitative or quantitative methods.Different forms of HIV-1 DNA in infected cells have been increasingly reported as biomarkers in HIV infection monitoring and AIDS treatment-related research.This article reviews the progress of HIV-1 DNA.