摘要
分子即时检测(point-of-care testing,POCT)技术具有灵敏度高、分析速度快、体积小、检测成本低廉等特点,在分子诊断领域受到广泛关注。近年来,分子POCT技术的发展与应用在应对新发、突发传染病,保护人类生命健康方面具有重大意义。介绍近五年来新兴的分子POCT技术,总结新兴分子POCT技术的最新研究进展及应用前景,分析POCT技术的优势与面临的挑战,探讨提高其检测灵敏度和选择性的技术策略。
The development and application of molecular point-of-care(POCT) technology has been noticed in recent years because of its high sensitivity, fast analysis speed, small size, and low detection cost. The development and application of molecular POCT technology is of great significance for protecting human life and health and coping with the threat of emerging and sudden infectious diseases. In this paper, several emerging molecular POCT technologies in the past five years, focusing on describing the latest research progress, and their application prospects were introduced. The advantages and challenges of molecular POCT were discussed, as well as the technical strategies to improve its detection sensitivity and selectivity.
作者
郭彦彤
刘仲明
张海燕
张宝
GUO Yan-tong;LIU Zhong-ming;ZHANG Hai-yan;ZHANG Bao(PLA Southern Theater Command General Hospital,Guangzhou 510010,China;BSL-3 Laboratory(Guangdong),Guangdong Provincial Key Laboratory of Tropical Disease Research,School of Public Health,Southern Medical University,Guangzhou 510515,China)
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2022年第9期50-57,共8页
China Biotechnology
基金
军队生物安全研究专项(19SWAQ24)
军队实验动物专项(SYDW[2020]18)资助项目。
关键词
POCT
分子诊断
新发传染病
生物安全
Point-of-care testing(POCT)
Molecular diagnostics
Emerging infectious diseases
Biosafety