普通环境设施内微屏障笼具饲养和维持清洁级小鼠初探

Preliminary Study on Raising and Maintaining Clean Mice with Microbarrier Cages in Conventional Environmental Facilities

目的探讨在普通环境设施内用微屏障笼具饲养和维持清洁级小鼠的可行性。方法在普通环境内采用正压微屏障笼具饲养和维持清洁级小鼠,其间以每个季度一次对微屏障内动物的微生物状况进行第三方抽样检测。结果自2020年6月开始用微屏障笼具饲养清洁级小鼠,至2022年7月文章成稿时,在连续两年的抽样检测中没有检出国家标准中要求清洁级小鼠必须排除的微生物。结论微屏障笼具可以在普通环境设施内实现清洁级小鼠的饲养和维持。

Objective To explore the feasibility of breeding cleaning degree mice with micro-barrier cages in conventional environmental facilities.Methods Clean-grade mice were housed and maintained in positive-pressure microbarrier cages in a general environment,during which third-party sampling of the microbial status of the animals in the microbarrier was performed on a quarterly frequency.Results From June 2020 to July 2022,no microorganisms that must be excluded by the national standard for cleaning degree mice have been detected in two consecutive years. Conclusion Micro-barrier cages can be usedto raise and maintain cleaning grade mice in conventional environmental facilities.