银杏叶活性成分的提取及在化妆品中的应用研究

Research on the extraction of active components from ginkgo biloba leaves and their application in cosmetics

以银杏叶为原料,采用溶剂法和超声法提取了银杏叶黄酮类物质,讨论了超声法提取银杏叶黄酮的影响因素。以70.00%乙醇水溶液为溶剂,以料液比、提取功率、提取时间为变量进行了单因素实验,得到银杏叶黄酮的最佳提取工艺:银杏叶粉末∶70.00%乙醇水溶液=1∶12、超声功率为480 W、超声时间为20 min,实验中最高提取率达到了88.07 mg/g。当选择银杏叶粉末∶70.00%乙醇水溶液=1∶14、超声功率为540 W、超声时间为20 min时,提取物对DPPH自由基的清除率最高,实验中最高清除率达到了93.46%。提取物对280~300 nm、360~400 nm的光波吸收明显,说明其对抵抗长波黑斑效应紫外线和中波红斑效应紫外线均有一定的贡献。实验表明,从银杏叶中提取的黄酮类物质具有较好的抗氧化和防晒功效,可以应用在化妆品中。

Ginkgo biloba leaves were used as raw materials to extract flavonoids from ginkgo biloba leaves by solvent extraction and ultrasonic extraction,and the factors influencing the ultrasonic extraction of flavonoids from ginkgo biloba leaves were discussed.The single factor experiment was conducted with 70.00%ethanol water solution as solvent,the ratio of material to liquid,the extraction power and the extraction time as variables,and the best extraction process of ginkgo leaf flavonoids was obtained:ginkgo leaf powder∶70.00%ethanol water solution=1∶12,ultrasonic power of 480 W,ultrasonic time of 20 min.The highest extraction rate reached 88.07 mg/g in the experiment.When ginkgo leaf powder∶70.00%ethanol solution=1∶14,ultrasonic power was 540 W,and ultrasonic time was 20 min,the highest DPPH radical scavenging rate of the extract was 93.46%in the experiment.The extract has obvious absorption of light waves of 280~300 nm and 360~400 nm,indicating that it has certain contribution to the resistance to long wave black spot effect ultraviolet and medium wave erythema effect ultraviolet.The experiment shows that flavonoids extracted from ginkgo biloba leaves have good antioxidant and sunscreen effects and can be used in cosmetics.