基于Au纳米颗粒负载NiMn-LDH@CuO NW/CuF的电化学免疫传感器检测癌胚抗原

Electrochemical Immunosensor Based on Au NP Loaded NiMn-LDH@CuO NW/CuF for Carcinoembryonic Antigen Detection

采用水热法在泡沫铜基底上生长纳米花状结构的双氢氧化物NiMn-层状双氢氧化物(LDH),并通过还原法负载Au纳米颗粒(NP),以CuO纳米线(NW)为自支撑模板,在泡沫铜上合成了Au NP负载的NiMn双氢氧化物(Au NP/NiMn-LDH@CuO NW/CuF)。利用扫描电子显微镜(SEM)和X射线衍射仪(XRD)对其形貌、结构和化学组分进行了表征;同时,基于Au NP/NiMn-LDH@CuO NW/CuF的纳米结构,构建了检测癌胚抗原(CEA)的电化学免疫传感器。循环伏安法(CV)以及电化学阻抗谱(EIS)相互验证了传感器的成功制备,并通过差分脉冲伏安法(DPV)研究了传感器的电化学性能。结果表明:基于Au NP/NiMn-LDH@CuO/CuF的无标记免疫传感器对CEA的线性检测范围为100 fg/mL~100 ng/mL,检测下限为53 fg/mL,对CEA也具有稳定的抗干扰性。

Nano-flower-like double hydroxide NiMn-layered double hydroxide(LDH)was grown on copper foam substrate by hydrothermal method,and Au nanoparticles(NPs)were loaded by reduction method.With Cu nanowires(NWs)were used as self-supporting templates,NiMn double hydroxide loaded by Au NPs(Au NPs/NiMn-LDH@CuO NWs/CuF)were synthesized on foamed copper foam substrate.The morphology,structure and chemical composition were characterized by scanning electron microscope(SEM)and X-ray diffractometer(XRD).Meanwhile,an electrochemical immunosensor for the detection of carcinoembryonic antigen(CEA)was constructed based on the nanostructure of Au NP/NiMn-LDH@CuO NW/CuF.Cyclic voltammetry(CV)and electrochemical impedance spectroscopy(EIS)mutually verified the successful fabrication of the sensor,and the electrochemical performance of the sensor was investigated by differential pulse voltammetry(DPV).The results show that the Au NPs/NiMn-LDH@CuO/CuF unlabeled immunosensor exhibits linear detection range of 100 fg/mL-100 ng/mL,detection limit of 53 fg/mL and stable anti-interference to CEA.