纳米银对连续三个世代褶皱臂尾轮虫的毒性效应

Toxic Effects of Silver Nanoparticles on Three Successive Generations of Brachionus plicatilis

为了探究纳米银(Ag NPs)长期暴露下对海洋浮游动物的毒性效应,本研究通过以海洋浮游动物褶皱臂尾轮虫(Brachionus plicatilis)为研究对象,研究了Ag NPs对连续三个世代褶皱臂尾轮虫的平均寿命、首次带卵时间、首次产幼时间和总后代数的影响,并通过产幼大小来明确非混交卵时期的发育情况以及通过摄食实验来探究Ag NPs对各世代轮虫能量摄入的影响。结果表明:Ag NPs对褶皱臂尾轮虫的24h-LC_(50)和48h-LC_(50)分别为18.7和3.4mg·L^(-1);不同浓度Ag NPs对各世代褶皱臂尾轮虫的生活史特征的影响存在显著的差异,且在同一世代中呈现出剂量-效应关系;在不同世代中,随着世代数的增加对其各生活史特征抑制效应显著增强。通过测量子代体长和进行摄食实验,结果发现能量的摄入不足和分配不均可能导致子代轮虫发育不良,进而导致其敏感性增强。

In order to explore the toxic effects of silver nanoparticles(Ag NPs)on marine zooplankton after long-term exposure.We studied the effects of silver nanoparticles(Ag NPs)on the average life span,time to the first batch of eggs,time to the first brood,and the total number of offspring of three successive generations of Brachionus plicatilis,and through the larva body length to clear the period of the development situation and by the feeding experiment to explore the effect of Ag NPs on the energy intake of rotifers in different generations.The results showed that the 24h-LC_(50) and 48h-LC_(50) were 18.7 and 3.4mg·L^(-1),respectively.There were significant differences in the effects of different concentrations of Ag NPs on the life history characteristics of Brachionus plicatilis in different generations,and there was a dose-response relationship in the same generation.In different generations,the inhibition effect on the life history characteristics of Brachionus plicatilis was significantly enhanced with the increase of the number of generations.By measuring the body length of the progeny and feeding experiments,the results showed that insufficient energy intake and uneven distribution may lead to the dysplasia of progeny rotifers,and thus enhance their sensitivity.