Collybistin对神经病理性疼痛的调控作用

The regulating effect of Collybistin on Neuropathic Pain

目的探讨collybistin在痛觉调控中的作用。方法利用免疫组织染色,观察collybistin在脊髓中的分布;利用行为学实验,评估collybistin在痛觉传递中的作用;利用电生理实验,研究collybistin对抑制性突触传递的影响。结果Collybistin分布于脊髓神经元中;通过转染针对collybistin的shRNA(shRNA-collybistin),发现shRNA-collybistin能够诱发正常小鼠的痛觉敏化(P<0.05);脊髓过表达collybistin,可明显缓解外周神经损伤诱发的痛觉敏化(P<0.05);ShRNA-collybistin也可明显降低脊髓背角浅层神经元中的微小抑制性突触后电流(miniature inhibitory postsynaptic currents;mIPSCs)的幅值和频率(P<0.05);脊髓过表达collybistin,可逆转外周神经损伤对mIPSCs的降低作用(P<0.05)。结论Collybistin参与外周神经损伤诱发小鼠痛觉敏化的过程。

Aim To investigate the role of collybistin in the regulation of pain transmission.Methods The distribution of collybistin in spinal cord was observed by immunohistochemical staining.The role of collybistin in pain transmission was evaluated by behavioral experiments.The effect of collybistin on inhibitory synaptic transmission was studied by electrophysiological experiments.Results Collybistin was distributed in spinal cord neurons;ShRNA-collybistin induced pain sensitization of intact mice(P<0.05).Overexpression of collybistin in spinal cord significantly alleviated pain sensitization induced by peripheral nerve injury(P<0.05).ShRNA-collybistin also significantly reduced the amplitudes and frequencies of miniature inhibitory postsynaptic currents(mIPSCs)in superficial neurons of spinal cord dorsal horn(P<0.05).Overexpression of collybistin in spinal cord could reverse the effects of peripheral nerve injury on mIPSCs(P<0.05).Conclusions Collybistin is involved in pain sensitization induced by peripheral nerve injury in mice.