载刺五加/桂皮紫萁海藻酸钠/羧甲基壳聚糖水凝胶伤口敷料的制备及其性能研究

Study on the Preparation and Properties of Acanthopanax senticosus/Cinnamomum cassia-loaded Sodium Alginate-carboxymethyl Chitosan Hydrogel Wound Dressing

为设计一种对伤口无损伤、抑菌抗炎效果好的伤口敷料,选择海藻酸钠(SA)与羧甲基壳聚糖(CMCS)为原料,通过抗拉伸强度优化SA/CMCS水凝胶的制备条件;然后添加刺五加/桂皮紫萁提取物,制备载刺五加/桂皮紫萁SA/CMCS水凝胶伤口敷料,并对其性质和功能进行研究。结果表明,SA/CMCS水凝胶最佳制备工艺为NaCl浓度0.8%,1-乙基-(3-二甲基氨基丙基)碳二亚胺盐酸盐/N-羟基琥珀酰亚胺(EDC/NHS)添加量2.25%,m(SA)∶m(CMCS)=1∶1,此时抗拉伸强度为0.884 MPa。红外光谱分析表明SA与CMCS为化学交联。载刺五加/桂皮紫萁SA/CMCS水凝胶伤口敷料孔隙率为(90.5±1.2)%,溶胀度为(133±2.7)%,药物累计释放率为(93.34±2.4)%,且具有良好缓释作用、抑菌抗炎性和生物相容性。制备的载刺五加/桂皮紫萁SA/CMCS水凝胶伤口敷料是一种良好的伤口敷料。

In order to design a wound dressing that does not damage to the wound, have good anti-bacterial and anti-inflammatory effect, sodium alginate(SA) and carboxymethyl chitosan(CMCS) were selected as raw materials, the preparation conditions of SA/CMCS hydrogel were optimized by tensile strength;Acanthopanax senticosus/Cinnamomum cassia extraction was compounded into hydrogel, to make Acanthopanax senticosus/Cinnamomum cassia-loaded SA/CMCS hydrogel wound dressing, the properties and functions were studied.The results showed that the optimal preparation process of SA/CMCS hydrogel was 0.8% NaCl, 2.25% EDC/NHS,m(SA)∶m(CMCS)=1∶1,under this condition, the tensile strength was 0.884 MPa.Infrared spectroscopy analysis showed that SA and CMCS was chemical cross-linking;the porosity of Acanthopanax senticosus/Cinnamomum cassia loaded SA/CMCS hydrogel was(90.5±1.2)%,the swelling was(133±2.7)%,the ratio of drug release was(93.34±2.4)%.At the same time, it showed good sustained release effect, antibacterial and anti-inflammatory effects, and excellent biocompatibility, as well.The results showed that the prepared Acanthopanax senticosus/Cinnamomum cassia-loaded SA-CMCS hydrogel could be used as a good wound dressing material.