激发波长和溶液浓度对罗丹明6G绝对量子产率的影响

Effects of Excitation Wavelength and Solution Concentration on the Absolute Quantum Yield of Rhodamine 6G

为了减小相对法量子产率测量过程中由于实验参数选择不合适引起的误差,采用高级荧光稳态瞬态测量系统和积分球相结合的装置测量了一系列浓度标准物质罗丹明6G溶液在不同激发波长下的绝对量子产率,研究了溶液浓度和激发波长对罗丹明6G绝对量子产率的影响。结果发现,当激发波长在483~488 nm之间,溶液浓度在7.5×10^(-7)~2.0×10^(-6)mol·L^(-1)范围时,绝对量子产率基本不变,数值为95%左右;而在其他激发波长以及溶液浓度时,绝对量子产率均降低。同时,在相对法量子产率测量过程中,标准物质激发波长和溶液浓度非常重要,选择不合适将会引起较大的测量误差。实验测试了不同溶液浓度和激发波长下罗丹明6G的绝对量子产率,可为其他物质相对法量子产率的测量提供参考。

In order to reduce the error caused by improper parameters in the measurement of relative quantum yield,the absolute quantum yield of standard substance Rhodamine 6 G solution with a series of concentrations at different excitation is measured by the instrument of advanced steady-state&time-resolved spectrofluorometer combined integrating sphere,and the effects of solution concentration and excitation wavelength on the absolute quantum yield of Rhodamine 6 G are studied.The results show that when the excitation wavelength is from 483 nm to 488 nm and the solution concentration is from 7.5×10^(-7) to 2.0×10^(-6)mol·L^(-1),the absolute quantum yield reaches about 95%,which is basically consistent with the literature reports.However,at other excitation wavelengths and solution concentrations,the absolute quantum yield decreases,thus improper selection will lead to larger errors in the measurement of the relative quantum yield.Otherwise,the absolute quantum yield of Rhodamine 6 G at different solution concentrations and excitation wavelength can provide an important reference for the measurement of relative quantum yield.