细胞与活体内活性氧成像分析的有机荧光探针

Versatile organic fluorescent probes for imaging reactive oxygen species in living cells and in vivo

活性氧(reactive oxygen species,ROS)是有氧代谢过程中形成的分子氧代谢物,参与细胞内多种生理和病理过程,与诸多疾病的发生发展密切相关.为研究ROS在相关生理病理事件中的作用,开发监测生物体内ROS的有效方法至关重要.基于探针的荧光成像技术因其高灵敏度和高时空分辨率而成为监测细胞和活体内生物活性分子的有力工具.本文从不同ROS有机荧光探针的构建出发,对近年来该类探针的研究进展进行了总结,重点对其设计思路、反应机理和生物应用进行了阐述.同时,也介绍了本课题组在该领域的研究成果.最后对未来挑战与发展方向进行了展望.

Reactive oxygen species(ROS)are oxygen metabolites formed during aerobic metabolism.They are implicated in a number of physiological and pathological processes in cells and are related to the occurrence and progression of various diseases.To study the role that ROS play in relevant physiopathological events,it is essential to develop effective methods to monitor ROS in living organisms.Fluorescent probe-based imaging techniques have emerged as a powerful tool for monitoring bioactive molecules in cells and in vivo,due to its high sensitivity and high spatiotemporal resolution.From the point of the construction of probes,this review systematically summarized the recent progress in the development of ROS organic fluorescent probes,with focus on the design strategies,reaction mechanisms and biological applications.Meanwhile,the research progress achieved by our group was also introduced.Finally,the challenges and future directions of this promising field were discussed.