微/纳米气泡水对盐胁迫下水稻幼苗的影响

Effects of Micro/nano Bubble Water on the Growth of Rice Seedlings under Salt Stress

为探究微/纳米气泡水增氧对盐胁迫下水稻幼苗生长的影响,以9311(模式种)和JX99(较耐盐)为供试材料,用Yoshida营养液+蒸馏水与Yoshida营养液+微/纳米气泡水培育水稻幼苗,再进行0.6%NaCl盐胁迫与无盐胁迫处理,测定水稻幼苗生长指标及生化指标。结果表明,无盐胁迫下,微/纳米气泡水增氧,9311水稻幼苗的根长、茎鲜重和茎基宽显著减小,分别减少24.87%、11.20%和12.50%,JX99的根长、叶片数显著增大,分别增加31.76%、21.88%,2个品种幼苗的丙二醛含量显著减小,分别减少41.19%、15.85%,2个品种的脯氨酸含量没有显著变化;0.6%盐胁迫下,微/纳米气泡水增氧,9311水稻幼苗的根长、茎鲜重、根鲜重显著减小,分别减少45.16%、46.81%和38.64%,JX99的茎长、根长、茎鲜重、根鲜重显著减小,分别减少28.09%、35.67%、67.97%和55.43%,2个品种幼苗的丙二醛显著增大,分别增加46.25%、36.11%,2个品种幼苗的脯氨酸含量显著增大,分别增加85.63%、131.64%。无盐胁迫时,微/纳米气泡水减缓2个品种幼苗细胞膜质过氧化程度,降低活性氧的伤害;0.6%盐胁迫下,微/纳米气泡水加剧2个品种幼苗细胞膜质过氧化程度,加剧活性氧的伤害,渗透胁迫也加剧。

The research aimed to study the effects of micro/nano bubble water on the growth of rice seedlings under salt stress.With rice variety 9311(model species)and JX99(relatively salt-tolerant)as test materials,the rice seedlings liquid cultivation experiment was conducted with Yoshida nutrient solution+distilled water and Yoshida nutrient solution+micro/nano bubble water,then they were treated with 0.6%NaCl salt stress and no salt stress.The growth characters and physiological and biochemical indexes of rice seedlings were determined.Results showed that under salt free stress,the root length,stem fresh weight and stem base width of 9311 rice seedlings decreased significantly by 24.87%,11.20%and 12.50%,the root length and leaf number of JX99 increased significantly by 31.76%,21.88%,the malondialdehyde content of the two varieties decreased significantly by 41.19%,15.85%,and the proline content of the two varieties did not change significantly;under 0.6%salt stress,the root length,stem fresh weight and root fresh weight of 9311 rice seedlings decreased significantly by 45.16%,46.81%and 38.64%.The stem length,root length,stem fresh weight and root fresh weight of JX99 increased significantly by 28.09%,35.67%,67.97%and 55.43%,the malondialdehyde contents of the two varieties increased significantly by 46.25%and 36.11%,the proline content of the two varieties increased significantly by 85.63%and 131.64%.Under salt free stress,micro/nano bubble water slowed down the degree of membrane peroxidation and reduced the damage of reactive oxygen species;under 0.6%salt stress,micro/nano bubble water exacerbated the degree of membrane peroxidation and the damage of reactive oxygen species and osmotic stress.