透骨香果实内源抑制物质研究

Study on Endogenous Inhibitory Substance in the Fruit of Gaultheria yunnanensis

[目的]研究透骨香果实(果皮和种子)中所含的内源抑制物质活性及其所存在部位。[方法]以白菜种子为试验对象,在不同温度、溶剂、浓度、浸提次数及时间的条件下对透骨香种子和果皮的浸提液进行生物活性测定。[结果]透骨香果实存在内源抑制物质,所得浸提液对试验种子的萌发和生长的抑制强弱为果皮浸提液>种子浸提液,水浸提液>甲醇浸提液;浸提液浓度越高,抑制越强;随着浸提次数增多,抑制作用逐渐减弱;浸提温度、提取时间对其有一定抑制作用。[结论]透骨香果实所含的内源抑制物质可能是种子萌发周期长、发芽率低的重要原因。

[Objective]To study the endogenous suppressor in Gaultheria yunnanensis fruit(pericarp and seed)and where its exist.[Method]Taking Chinese cabbage seeds as the test object,the biological activities of the extracts from the seeds and pericarp of Gaultheria yunnanensis were measured under different conditions of temperature,solvent,concentration,extraction times and extraction time.[Result]There were endogenous inhibitory substances in the fruit of Gaultheria yunnanensis,and the inhibition effect of the obtained extract on the germination and growth of the test seeds was the peel extract>the seed extract,aqueous extract>methanol extract.The higher the extract concentration,the stronger the inhibition;with the increase of extraction times,the inhibitory effect gradually weakened;the extraction temperature and extraction time had a certain inhibitory effect on it.[Conclusion]The endogenous inhibitory substances in seeds of Gaultheria yunnanensis may be an important reason for the long seed cycle and low germination rate.