非小细胞肺癌组织miR-148a-3p、Hint1 mRNA表达水平与患者预后的相关性分析

Correlation between the expression levels of miR-148a-3p,Hint1 mRNA in non small cell lung cancer tissue and the prognosis of patients

目的检测非小细胞肺癌组织中微小RNA-148a-3p(miR-148a-3p)、组氨酸三聚体核苷结合蛋白1(Hint1)信使核糖核酸(mRNA)表达水平,并分析其与患者预后的关系。方法选取2014年6月—2016年10月于浙江省台州医院就诊的86例非小细胞肺癌患者,术中收集患者肺癌组织和配对的癌旁组织,采用实时荧光定量PCR法检测所有组织miR-148a-3p、Hint1 mRNA表达水平,分析肺癌组织miR-148a-3p、Hint1 mRNA表达水平与患者临床病理特征的关系及二者之间的相关性,Kaplan-Meier法分析肺癌组织miR-148a-3p、Hint1 mRNA表达水平与患者5年总生存率的关系;多因素logistic回归分析影响非小细胞肺癌患者预后的危险因素。结果肺癌组织miR-148a-3p表达水平高于癌旁组织(P<0.05),Hint1 mRNA表达水平低于癌旁组织(P<0.05);无淋巴结转移患者肺癌组织miR-148a-3p表达水平低于有淋巴结转移患者,Hint1 mRNA表达水平高于有淋巴结转移患者(P<0.05);I期~Ⅱ期患者肺癌组织miR-148a-3p表达水平低于Ⅲ期患者,Hint1 mRNA表达水平高于Ⅲ期患者(P<0.05);肺癌组织miR-148a-3p与Hint1 mRNA表达水平成负相关(P<0.05);肺癌组织miR-148a-3p高表达、Hint1 mRNA低表达是非小细胞肺癌患者不良预后的危险因素(P<0.05)。结论非小细胞肺癌组织中miR-148a-3p表达上调、Hint1 mRNA表达下调,与患者临床病理特征和预后密切相关,可能成为新的肺癌治疗靶点。

Objective To detect the expression levels of microRNA-148a-3p(miR-148a-3p)and histidine triad nucleotide binding protein 1(Hint1)messenger RNA(mRNA)in non small cell lung cancer and analyze their relationship with the prognosis of patients.Methods Eighty six patients with non small cell lung cancer treated in Taizhou Hospital from June 2014 to October 2016 were selected.The lung cancer tissues and paired adjacent tissues were collected during the operation.The expression levels of miR-148a-3p and Hint1 mRNA in all tissues were detected by real-time fluorescence quantitative PCR.The relationship between the expression levels of miR-148a-3p and Hint1 mRNA in lung cancer tissue and the clinicopathological features of patients and the correlation between them were analyzed.The relationship between the expression levels of miR-148a-3p and Hint1 mRNA in lung cancer and the 5-year overall survival rate of patients was analyzed by Kaplan-Meier method.Multivariate logistic regression analysis was conducted on the risk factors affecting the prognosis of patients with non small cell lung cancer.Results The expression level of miR-148a-3p in lung cancer tissues was higher than that in adjacent tissues(P<0.05),and the expression level of Hint1 mRNA was lower than that in adjacent tissues(P<0.05);the expression level of miR-148a-3p in lung cancer without lymph node metastasis was lower than that in patients with lymph node metastasis,and the expression level of Hint 1 mRNA was higher than that in patients with lymph node metastasis(P<0.05);the expression level of miR-148a-3p in stageⅠ-Ⅱpatients was lower than that in stageⅢpatients,and the expression level of Hint1 mRNA was higher than that in stageⅢpatients(P<0.05);there was a negative correlation between miR-148a-3p and Hint1 mRNA expression in lung cancer(P<0.05);high expression of lung cancer tissue miR-148a-3p and low expression of Hint1 mRNA were risk factors for poor prognosis in patients with non small cell lung cancer(P<0.05).Conclusion The expression of miR-148a-3p was up-regulated and Hint1 mRNA was down regulated in non small cell lung cancer tissue,which are closely related to the clinicopathological features and prognosis of patients,and may become new targets for lung cancer treatment.