弥漫大B细胞淋巴瘤患者血清氨基酸谱的靶向代谢组学研究

Targeted metabolomics study of serum amino acid profile in diffuse large B cell lymphoma

目的探究弥漫大B淋巴瘤(dffuse large B lymphoma,DLBCL)患者与正常人是否存在不同血清氨基酸谱。方法使用超高效液相色谱-串联质谱(ultra performance liquid chromatography-tandem mass,UPLC-MS/MS)技术对上述DLBCL组患者和对照组进行靶向氨基酸定量分析。结果在DLBCL组肌氨酸、丙氨酸、2-氨基丁酸、丝氨酸、异亮氨酸、鸟氨酸、苯丙氨酸、酪氨酸比对照组呈上升趋势(P<0.05),赖氨酸、甘氨酸、苏氨酸、亮氨酸、天冬酰胺、组氨酸、瓜氨酸、缬氨酸、色氨酸呈下降趋势(P<0.05);PLS-DA模型及ROC曲线下面积筛选出肌氨酸区分DLBCL与对照组能力最强;肌氨酸和赖氨酸可作为联合血清标志物,提高诊断准确率。结论DLBCL患者血清氨基酸谱发生明显改变,肌氨酸可以作为DLBCL的血清标志物,肌氨酸、赖氨酸联合氨基酸有望成为诊断工具。

Objective To investigate whether there is difference in serum amino acid profile between patients with diffuse large B-cell lymphoma and patients with normal health.Methods Ultra-performance liquid chromatography-tandem mass(UPLC-MS/MS)was used for the quantitative analysis of targeted amino acids in the above two groups.Results The levels of sarcosine,alanine,2-aminobutyric acid,serine,isoleucine,ornithine,phenylalanine and tyrosine in DLBCL group were higher than those in the control group(P<0.05);the levels of lysine,glycine,threonine,leucine,asparagine,histidine,citrulline,valine,tryptophan showed a decreasing trend(P<0.05).The PLS-DA model and area under ROC curve screen Sar has the strongest ability to distinguish DLBCL from control group.The combined application of sarcosine and lysine can be biomarkers to improve the diagnostic accuracy.Conclusion Serum amino acid profiles of DLBCL patients were significantly changed.Sarcosine can be used as a serum marker of DLBCL.The combination of sarcosine and lysine amino acids is expected to be a diagnostic tool.