基于KASP技术的SNP标记用于西瓜品种指纹图谱构建和种子纯度检测

Application of SNP markers based on RASP technology in fingerprint construction and seed purity detection of watermelon varieties

随着SNP标记在育种工作中的应用,KASP技术因其低成本、高通量、高准确性等优势,在作物指纹图谱构建及种子纯度检测等方面具有很高的应用价值。已报道的西瓜品种分子鉴定和指纹图谱多采用SSR标记,试验过程较为繁琐,效率较低。本研究利用32对核心SNP标记,采用KASP技术对54份包括野生型、东亚型和美洲型等不同农艺性状的西瓜自交系材料和25份西瓜杂交种进行了基因分型,明确了自交系材料之间遗传距离和遗传背景,为西瓜育种提供了方向,并构建了苏梦和苏创系列西瓜品种的指纹图谱。从32对引物中筛选出的2对引物可用于苏梦5号、苏梦6号、苏梦7号、苏梦9号、苏创3号、苏创4号和苏创5号的种子纯度检测,这为西瓜品种及种子纯度鉴定提供了简便、快捷、高效的方法。

With the application of SNP markers in breeding,KASP technology is of great value in crop fingerprint construction and seed purity detection due to its low cost,high throughput and high accuracy.SSR markers are commonly used for molecular identification and fingerprint of watermelon varieties,but the test process is complicated and inefficient.Fifty-four watermelon inbred lines including wild type,East Asian type and American type,and 25 watermelon hybrids with distinct agronomic traits were genotyped using KASP technology and 32 pairs of core SNP markers.The genetic distance and genetic background between the inbred lines were revealed,which provided a direction for watermelon breeding,and the fingerprints of Sumeng and Suchuang brand watermelon varieties were constructed.Two pairs of primers screened from 32 pairs of primers could be used for seed purity detection of Sumeng No.5,Sumeng No.6,Sumeng No.7,Sumeng No.9,Suchunag No.3,Suchuang No.4 and Suchuang No.5,a simple,fast and efficient method for variety identification and seed purity testing of watermelon.