青蒿紫云膏治疗日光疹的网络荟萃分析与网络药理学研究

Qinghao Ziyun Ointment in the Treatment of Solar Dermatitis Based on the Network Meta-Analysis and Network Pharmacology

目的基于网络荟萃分析和网络药理学,探讨青蒿紫云膏治疗日光疹的临床疗效和潜在作用机制。方法采用计算机检索PubMed,Embase,The Cochrane Library,CNKI,VIP,WanFang,CBM数据库自建库起至2021年12月31日关于青蒿紫云膏治疗日光疹的随机对照试验,根据纳入与排除标准提取相关数据,采用R 4.1.2软件和ADDIS 1.16软件进行网络荟萃分析,采用TCMSP和Swiss TargetPrediction数据库筛选药物有效成分及靶点,采用GeneCards数据库获得疾病靶点,采用Venny 2.1线上工具获得药物-疾病共有靶点,采用String数据库构建蛋白互作网络,对药物-疾病共有靶点进行GO生物功能富集分析和KEGG通路富集分析。结果网络荟萃分析共纳入7篇文献,涉及629例患者。分析结果显示,以青蒿+紫草为君药与H_(1)受体阻断剂+羟氯喹+(维生素E+维生素C+烟酰胺)治疗效果无显著差异[OR=0.91,95%CI(0.016,55.00)];以青蒿为君药与H_(1)受体阻断剂治疗效果无显著差异[OR=0.30,95%CI(0.058,1.4)]。网络药理学筛选出有效成分60个、靶点1335个、疾病靶点466个,药物-疾病共有靶点109个,关键靶点20个。GO生物功能富集分析主要集中于细胞组分和生物过程;KEGG通路富集分析主要影响肿瘤坏死因子、白细胞介素17、脂质与动脉粥样硬化、细胞衰老等信号通路。结论青蒿紫云膏治疗日光疹临床疗效较好,与H_(1)受体阻断剂单用或联用羟氯喹+维生素类制剂的临床疗效相似,主要通过抑制炎性反应、抗细胞衰老等通路发挥作用。

Objective To investigate the clinical efficacy and potential mechanism of Qinghao Ziyun Ointment in the treatment of solar dermatitis based on the network Meta-analysis and network pharmacology.Methods The randomized controlled trials(RCTs)of the treatment of solar dermatitis with Qinghao Ziyun Ointment in the PubMed,Embase,The Cochrane Library,CNKI,VIP,WanFang,CBM and other databases from the inception to December 31,2021 were searched by the computer.The relevant data were extracted according to the inclusion and exclusion criteria.The R 4.1.2 and ADDIS 1.16 softwares were used to do the network Meta-analysis,the TCMSP and Swiss TargetPrediction databases were used to screen the active components of drugs and targets,the GeneCards database was used to obtain targets of disease,the Venny 2.1 online tool was used to obtain drug-disease common targets,and the String database was used to construct the protein-protein interaction(PPI)network.The GO biological function enrichment analysis and KEGG pathways enrichment analysis were conducted for the drug-disease common targets.Results Seven studies were included in the network Meta-analysis,involving 629 patients.The results showed that the efficacy of Artemisiae Annuae Herba+Arnebiae Radix as the sovereign drugs was similar to that of H_(1)-receptor blockers+hydroxychloroquine+(vitamin E+vitamin C+nicotinamide),with no statistical difference[OR=0.91,95%CI(0.016,55.00)].The results also showed that the efficacy of Artemisiae Annuae Herba as the sovereign drug was similar to that of H_(1)-receptor blockers,with no statistical difference[OR=0.30,95%CI(0.058,1.4)].A total of 60 active components,1335 targets of active components,466 targets of disease,109 drug-disease common targets and 20 key targets were screened in the network pharmacology.The results of GO biological function enrichment analysis showed that Qinghao Ziyun Ointment in the treatment of solar dermatitis mainly focuses on the cellular components and biological processes.The results of KEGG pathways enrichment analysis showed that Qinghao Ziyun Ointment in the treatment of solar dermatitis mainly involved in the tumor necrosis factor(TNF),interleukin 17(IL-17),lipid and atherosclerosis and cellular senescence signal pathways.Conclusion Qinghao Ziyun Ointment is effective in the treatment of solar dermatitis,its clinical efficacy is similar to that of H_(1)-receptor blockers alone or combination with hydroxychloroquine+vitamins,mainly through the inhibition of inflammatory response,anti-cellular senescence and other pathways.