基于信息熵赋权法和正交试验优选乌头桂枝汤提取工艺

Optimization of Extraction Process of Wutou Guizhi Decoction Based on Information Entropy Weighting and Orthogonal Test

目的 基于信息熵赋权法优选乌头桂枝汤提取工艺。方法 以药物浸泡时间、提取时间、加水量为影响因素,以芍药苷、甘草苷、甘草酸单铵盐、单酯型生物碱、桂皮醛含量为评价指标,采用信息熵赋权法确定上述指标的权重系数,采用L_(9)(3^(4))正交试验优选提取工艺。结果 最佳工艺为加10倍量水浸泡1.0 h,提取时间1.0 h,每1 mL平均含芍药苷、甘草苷、单酯型生物碱、甘草酸单铵盐、桂皮醛分别为0.054 7,0.023 0,0.003 4,0.037 7,0.089 1 mg,平均综合评分为0.126 5。结论 该方法工艺合理、结果稳定,可用于乌头桂枝汤的提取。

ObjectiveTo optimize the extraction process of Wutou Guizhi Decoction based on the information entropy weighting method.MethodsThe extraction process was optimized by the L_(9)(3^(4)) orthogonal test with the soaking time of drugs,extraction time and the amount of water addition as the influencing factors,with the contents of paeoniflorin,liquiritin,glycyrrhizic acid ammonium salt,monoester alkaloids and cinnamaldehyde as the evaluation indexes,and the weight coefficients of the above indexes were determined by the information entropy weighting method.ResultsThe optimal process was as follows:the drugs were soaked for 1. 0 h with 10 times the amount of water,and extracting for 1. 0 h. The average contents of paeoniflorin,liquiritin,monoester alkaloids,glycyrrhizic acid ammonium salt and cinnamaldehyde in per 1 mL of extract of Wutou Guizhi Decoction were 0. 054 7,0. 023 0,0. 003 4,0. 037 7 and 0. 089 1 mg respectively,with the average comprehensive score of 0. 126 5.ConclusionThe method is reasonable and stable,which can be used for the extraction of Wutou Guizhi Decoction.