SP-D、CXCL13、lncRNA PVT1在老年COPD患者中的表达及与肺功能指标的相关性

The expression of SP-D,CXCL13 and lncRNA PVT1 in elderly COPD patients and their correlations with lung function indexes

目的探讨老年慢性阻塞性肺疾病(COPD)患者血清表面活性蛋白D(SP-D)及趋化因子配体13(CXCL13)水平、长链非编码RNA浆细胞瘤转化迁移基因1(lncRNA PVT1)表达,以及三者与肺功能的相关性。方法选择2019年3月至2021年5月湖北省武汉市优抚医院收治的300例老年COPD患者为研究对象,其中135例急性加重期患者纳入急性加重期组,165例稳定期患者纳入稳定期组,另选择65例体检健康者作为对照组。采用比色夹心酶联免疫吸附试验检测受试者血清SP-D、CXCL13水平,采用实时荧光定量聚合酶链反应检测lncRNA PVT1表达,FGC-A肺功能测试仪检测肺功能指标,分析SP-D、CXCL13、lncRNA PVT1与肺功能指标的相关性。采用受试者工作特征(ROC)曲线分析SP-D、CXCL13、lncRNA PVT1鉴别COPD急性加重期和稳定期的效能。结果急性加重期组、稳定期组血清SP-D、CXCL13水平及lncRNA PVT1表达高于对照组,第1秒用力呼气容积(FEV_(1))、FEV_(1)与用力肺活量(FVC)比值(FEV_(1)/FVC)、FEV_(1)占预计值百分数(FEV_(1)%pred)低于对照组,差异有统计学意义(P<0.05)。COPD患者血清SP-D、CXCL13水平及lncRNA PVT1表达均与FEV_(1)、FEV_(1)/FVC、FEV_(1)%pred呈负相关(P<0.05)。SP-D、CXCL13、lncRNA PVT1鉴别COPD急性加重期和稳定期的曲线下面积(AUC)分别为0.707、0.671、0.696,联合3项指标后AUC达0.878,大于SP-D、CXCL13、lncRNA PVT1单独检测的AUC(Z=5.922、6.741、6.470,P<0.05)。结论老年COPD患者血清SP-D、CXCL13水平及lncRNA PVT1表达均升高,且与COPD急性加重及肺功能下降有关。

Objective To investigate the relationships between serum levels of surface active protein D(SP-D),chemokine ligand 13(CXCL13),the expression of long chain-non-coding RNA plasmocytoma ribocytoma transferable migration gene 1(lncRNA PVT1)and lung function indexes in elderly patients with chronic obstructive pulmonary disease(COPD).Methods A total of 300 elderly COPD patients admitted to Wuhan Youfu Hospital from March 2019 to May 2021 were selected,including 135 patients in acute exacerbation stage(acute exacerbation stage group),165 patients in stable stage group(stable stage group),and 65 physical examination volunteers as control group.Serum levels of SP-D and CXCL13 were detected by colorimetric sandwich enzyme-linked immunosorbent assay,lncRNA PVT1 expression were detected by real-time fluorescence quantitative polymerase chain reaction,and FGC-A pulmonary function tester was used to measure lung function indexes.The correlations between SP-D,CXCL13,lncRNA PVT1 and lung function indexes were analyzed.Receiver operating characteristic(ROC)curve was used to analyze the value of SP-D,CXCL13 and lncRNA PVT1 in differentiating COPD acute exacerbation from stable stage.Results The serum levels of SP-D,CXCL13 and lncRNA PVT1 expression in acute exacerbation stage group and stable stage group were higher than those in control group,while forced expiratory volume in 1 second(FEV_(1)),ratio of FEV_(1)to forced vital capacity(FEV_(1)/FVC)and percentage of FEV_(1)to predicted value(FEV_(1)%pred)were lower than those in control group(P<0.05).Serum levels of SP-D,CXCL13 and lncRNA PVT1 expression correlated negatively with FEV_(1),FEV_(1)/FVC and FEV_(1)%pred in COPD patients(P<0.05).The area under the curve(AUC)of SP-D,CXCL13,lncRNA PVT1 in differentiating COPD acute exacerbation from stable stage were 0.707,0.671,0.696 respectively,the AUC of combining the three indexes was 0.878,which was grater than SP-D,CXCL13 and lncRNA PVT1 alone(Z=5.922,6.741,6.470,P<0.05).Conclusion The serum levels of SP-D,CXCL13 and lncRNA PVT1 expression in elderly COPD patients increase,which associate with COPD acute exacerbation and decreased lung function.